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Literature summary for 1.1.1.4 extracted from

  • Crow, V.L.
    Properties of 2,3-butanediol dehydrogenase from Lactococcus lactis subsp. lactis in relation to citrate fermentation (1990), Appl. Environ. Microbiol., 56, 1656-1665.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 enzyme 1 and 2, gel filtration Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 2,3-butanediol dehydrogenase 1 and 2
-
Lactococcus lactis
-
 subsp. lactis D10
-

Purification (Commentary)

Purification (Comment) Organism
2,3-butanediol dehydrogenase 1 and 2 Lactococcus lactis
partially Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1
-
 acetoin, enzyme 1 Lactococcus lactis
7.55
-
 acetoin, enzyme 2 Lactococcus lactis
35.55
-
 diacetyl, enzyme 2 Lactococcus lactis
35.6
-
 diacetyl, enzyme 1 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanediol + NAD+
-
 Lactococcus lactis acetoin + NADH
-
 r
acetoin + NADH + H+
-
 Lactococcus lactis 2,3-butanediol + NAD+ enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers r
diacetyl + NADH
-
 Lactococcus lactis 2,3-butanediol + NAD+ enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 substrate reduction, enzyme 1 Lactococcus lactis
6.1
-
 substrate reduction, enzyme 2 Lactococcus lactis
8.5
-
 substrate oxidation, enzyme 2 Lactococcus lactis
10
-
 substrate oxidation, enzyme 1 Lactococcus lactis