Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM105 cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | noncompetitive inhibition | Escherichia coli | |
D-2-hydroxyglutaric acid | competitive inhibition | Escherichia coli | |
glycine | - |
Escherichia coli | |
L-serine | noncompetitive inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
2-oxoglutarate | at pH 7.5 and 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-hydroxyglutarate + NAD+ | Escherichia coli | - |
2-oxoglutarate + NADH + H+ | - |
r | |
2-oxoglutarate + NADH + H+ | Escherichia coli | - |
(R)-2-hydroxyglutarate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and 5'-AMP-Sepharose affinity column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.27 | - |
crude extract, at pH 7.5 and 37°C | Escherichia coli |
9.7 | - |
after 3fold purification, at pH 7.5 and 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-hydroxyglutarate + NAD+ | - |
Escherichia coli | 2-oxoglutarate + NADH + H+ | - |
r | |
2-oxoglutarate + NADH + H+ | - |
Escherichia coli | (R)-2-hydroxyglutarate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alphaKG reductase | - |
Escherichia coli |
serA | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | the enzyme remains stable after 3 min incubation between 0 and 40°C, while 3 min at 45, 50 and 60°C leads to about 40, 70, and 100% loss of activity | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.3 | - |
2-oxoglutarate | at pH 7.5 and 37°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
L-serine | at pH 7.5 and 37°C | Escherichia coli | |
0.37 | - |
D-2-hydroxyglutaric acid | at pH 7.5 and 37°C | Escherichia coli | |
12.7 | - |
3-phosphoglycerate | at pH 7.5 and 37°C | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0076 | - |
at pH 7.5 and 37°C | Escherichia coli | L-serine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
380 | - |
2-oxoglutarate | at pH 7.5 and 37°C | Escherichia coli |