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Literature summary for 1.1.1.393 extracted from

  • Chen, M.; Drury, J.E.; Christianson, D.W.; Penning, T.M.
    Conversion of human steroid 5beta-reductase (AKR1D1) into 3beta-hydroxysteroid dehydrogenase by single point mutation E120H: example of perfect enzyme engineering (2012), J. Biol. Chem., 287, 16609-16622.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.393

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Ruminococcus sp. 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+ Ruminococcus sp.
-
 3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
-
 ?
3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+ Ruminococcus sp. PO1-3
-
 3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Ruminococcus sp.
-
 isolated from human intestine
-
Ruminococcus sp. PO1-3
-
 isolated from human intestine
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially, separation from 7beta-hydroxysteroid dehydrogenase, EC 1.1.1.201, by hydrophobic interaction chromatography Ruminococcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Ruminococcus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
-
 Ruminococcus sp. 3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
-
 ?
3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
-
 Ruminococcus sp. PO1-3 3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
3beta-hyoxysteroid dehydrogenase
-
 Ruminococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ruminococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 assay at Ruminococcus sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Ruminococcus sp.

General Information

General Information Comment Organism
metabolism the organism also possesses 3beta-hyoxysteroid dehydrogenase activity that is active with the same substrate as the 3beta-hyoxysteroid dehydrogenase but is specific for 7beta-hydroxy groups, overview Ruminococcus sp.