Cloned (Comment) | Organism |
---|---|
gene Elen_0198, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli | Eggerthella lenta |
gene Elen_1325, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli | Eggerthella lenta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.63 | - |
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid | pH 7.0, 37°C | Eggerthella lenta | |
2.66 | - |
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid | pH 7.0, 37°C | Eggerthella lenta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+ | Eggerthella lenta | - |
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+ | - |
? | |
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+ | Eggerthella lenta ATCC 25559 | - |
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eggerthella lenta | C8WGQ3 | gene Elen_1325 | - |
Eggerthella lenta | C8WJW0 | gene Elen_0198 | - |
Eggerthella lenta ATCC 25559 | C8WGQ3 | gene Elen_1325 | - |
Eggerthella lenta ATCC 25559 | C8WJW0 | gene Elen_0198 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+ | - |
Eggerthella lenta | 3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+ | - |
? | |
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+ | - |
Eggerthella lenta ATCC 25559 | 3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSDH | - |
Eggerthella lenta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Eggerthella lenta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.73 | - |
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid | pH 7.0, 37°C | Eggerthella lenta | |
29.71 | - |
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid | pH 7.0, 37°C | Eggerthella lenta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Eggerthella lenta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Eggerthella lenta |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids | Eggerthella lenta |
physiological function | the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides | Eggerthella lenta |