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Literature summary for 1.1.1.391 extracted from

  • Devlin, A.; Fischbach, M.
    A biosynthetic pathway for a prominent class of microbiota-derived bile acids (2015), Nat. Chem. Biol., 11, 685-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Elen_0198, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli Eggerthella lenta
gene Elen_1325, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli Eggerthella lenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.63
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid pH 7.0, 37°C Eggerthella lenta
2.66
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid pH 7.0, 37°C Eggerthella lenta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+ Eggerthella lenta
-
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+ Eggerthella lenta ATCC 25559
-
3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Eggerthella lenta C8WGQ3 gene Elen_1325
-
Eggerthella lenta C8WJW0 gene Elen_0198
-
Eggerthella lenta ATCC 25559 C8WGQ3 gene Elen_1325
-
Eggerthella lenta ATCC 25559 C8WJW0 gene Elen_0198
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
-
Eggerthella lenta 3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
?
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid + NADH + H+
-
Eggerthella lenta ATCC 25559 3beta,12alpha-dihydroxy-5-beta-cholan-24-oic acid + NAD+
-
?

Synonyms

Synonyms Comment Organism
3beta-HSDH
-
Eggerthella lenta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Eggerthella lenta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.73
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid pH 7.0, 37°C Eggerthella lenta
29.71
-
12alpha-hydroxy-3-oxo-5-beta-cholan-24-oic acid pH 7.0, 37°C Eggerthella lenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Eggerthella lenta

Cofactor

Cofactor Comment Organism Structure
NADH
-
Eggerthella lenta

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids Eggerthella lenta
physiological function the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides Eggerthella lenta