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Literature summary for 1.1.1.391 extracted from

  • Edenharder, R.; Pfutzner, A.; Hammann, R.
    Characterization of NAD-dependent 3alpha- and 3beta-hydroxysteroid dehydrogenase and of NADP-dependent 7beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus (1989), Biochim. Biophys. Acta, 1004, 230-238.
    View publication on PubMed

General Stability

General Stability Organism
DTT stabilizes, while ETA has no influence on enzyme stability Blautia producta

Inhibitors

Inhibitors Comment Organism Structure
Bile acids specific enzymatic activities are significantly reduced when bacteria are grown in the presence of 3-oxo bile acids, while other bile acids are ineffective Blautia producta

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Blautia producta 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
132000
-
-
Blautia producta

Organism

Organism UniProt Comment Textmining
Blautia producta
-
-
-
Blautia producta K2/25-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography and gel filtration Blautia producta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid Blautia producta
0.38
-
pH 9.5, 25°C, substrate 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid Blautia producta
0.59
-
pH 9.5, 25°C, substrate 3,12-dioxo-5beta-cholan-24-oic acid Blautia producta
1
-
pH 9.5, 25°C, substrate 7alpha,12alpha-3-oxo-dihydroxy-5beta-cholan-24-oic acid Blautia producta
2.12
-
pH 9.5, 25°C, substrate 3,7,12-trioxo-5beta-cholan-24-oic acid Blautia producta
2.35
-
pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid Blautia producta
2.45
-
pH 9.5, 25°C, substrate 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid Blautia producta
2.49
-
pH 9.5, 25°C, substrate 12alpha-hydroxy-3-oxo-5beta-cholate Blautia producta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
Blautia producta 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ sole pruduct ?
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
Blautia producta 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+
-
?
3,7-dioxo-5beta-cholan-24-oic acid + NADH + H+
-
Blautia producta 3beta-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD+
-
?
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
Blautia producta 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
?
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
Blautia producta 3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
?
additional information no activity with 3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid, 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid, 3alpha,7beta-dihydroxytaureate, 7,12-dioxo-5beta-cholanate, 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate and 3alpha-hydroxy-7,12-dioxo-5beta-cholanate Blautia producta ?
-
?

Synonyms

Synonyms Comment Organism
3beta-HSDH
-
Blautia producta
NAD-dependent 3beta-hydroxysteroid dehydrogenase
-
Blautia producta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Blautia producta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
crude cell extract, prepared in 0.1 M Tris-HCl, pH 8.5, 1.0 mM EDTA, and 1.0 mM DTT in absence of bile acid substrate, stable for several hours and detectable for at least 36 h Blautia producta
37
-
pH 9.5, activity is reduced to about 20% within 1 h Blautia producta
50
-
pH 9.5, 10 min, loss of 75% activity, the activity is completely lost within about 30 h Blautia producta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Blautia producta

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Blautia producta