General Stability | Organism |
---|---|
DTT stabilizes, while ETA has no influence on enzyme stability | Blautia producta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bile acids | specific enzymatic activities are significantly reduced when bacteria are grown in the presence of 3-oxo bile acids, while other bile acids are ineffective | Blautia producta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Blautia producta | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
132000 | - |
- |
Blautia producta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blautia producta | - |
- |
- |
Blautia producta K2/25-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange chromatography and gel filtration | Blautia producta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid | Blautia producta |
0.38 | - |
pH 9.5, 25°C, substrate 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid | Blautia producta |
0.59 | - |
pH 9.5, 25°C, substrate 3,12-dioxo-5beta-cholan-24-oic acid | Blautia producta |
1 | - |
pH 9.5, 25°C, substrate 7alpha,12alpha-3-oxo-dihydroxy-5beta-cholan-24-oic acid | Blautia producta |
2.12 | - |
pH 9.5, 25°C, substrate 3,7,12-trioxo-5beta-cholan-24-oic acid | Blautia producta |
2.35 | - |
pH 9.5, 25°C, substrate 3,7-dioxo-5beta-cholan-24-oic acid | Blautia producta |
2.45 | - |
pH 9.5, 25°C, substrate 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid | Blautia producta |
2.49 | - |
pH 9.5, 25°C, substrate 12alpha-hydroxy-3-oxo-5beta-cholate | Blautia producta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
Blautia producta | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | sole pruduct | ? | |
3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+ | - |
Blautia producta | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
3,7-dioxo-5beta-cholan-24-oic acid + NADH + H+ | - |
Blautia producta | 3beta-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
Blautia producta | 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
Blautia producta | 3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
additional information | no activity with 3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid, 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid, 3alpha,7beta-dihydroxytaureate, 7,12-dioxo-5beta-cholanate, 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate and 3alpha-hydroxy-7,12-dioxo-5beta-cholanate | Blautia producta | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSDH | - |
Blautia producta |
NAD-dependent 3beta-hydroxysteroid dehydrogenase | - |
Blautia producta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Blautia producta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
crude cell extract, prepared in 0.1 M Tris-HCl, pH 8.5, 1.0 mM EDTA, and 1.0 mM DTT in absence of bile acid substrate, stable for several hours and detectable for at least 36 h | Blautia producta |
37 | - |
pH 9.5, activity is reduced to about 20% within 1 h | Blautia producta |
50 | - |
pH 9.5, 10 min, loss of 75% activity, the activity is completely lost within about 30 h | Blautia producta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Blautia producta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Blautia producta |