Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-malate | activates the enzyme in mitochondria | Amaranthus cruentus | |
NAD+ | activates the enzyme in mitochondria | Amaranthus cruentus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Amaranthus cruentus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | in the pre-anthesis phase NAD-ME isoform with MW 115 kDa occurs in cytosol of mesophyll and bundle sheath cells of control and drought-exposed plants. In mesophyll cells 100% activity is found in cytosol fractions, and about 9.4% of the total NAD-ME activity is localized in cytosol of bundle sheath cells | Amaranthus cruentus | 5829 | - |
mitochondrion | one of NAD-ME isoforms with MW 110 kDa is located in mitochondria of bundle sheath cells of control and drought-exposed plants, and another isoform of MW 121 kDa is formed in mitochondria of bundle sheath cells under influence of drought. After resuming watering the 121 kDa isoform disappears again. About 90.6% of the total NAD-ME activity is localized in mitochondrial stroma of bundle sheath cells | Amaranthus cruentus | 5739 | - |
additional information | several molecular forms of NAD-ME with different subcellular localization patterns in the studied phases of amaranth ontogenesis. No enzyme activity is observed experimentally in chloroplasts of mesophyll and bundle sheath cells | Amaranthus cruentus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates the enzyme in mitochondria, kinetics, overview | Amaranthus cruentus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
mitochondrial isozyme of bundle sheath cells of control and drought-exposed plants | Amaranthus cruentus |
115000 | - |
cytosolis isozyme of mesophyll and bundle sheath cells of control and drought-exposed plants | Amaranthus cruentus |
121000 | - |
mitochondrial isozyme of bundle sheath cells of drought-exposed plants | Amaranthus cruentus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Amaranthus cruentus | - |
pyruvate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amaranthus cruentus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bundle sheath cell | - |
Amaranthus cruentus | - |
leaf | - |
Amaranthus cruentus | - |
mesophyll cell | in mesophyll cells, 100% activity is found in cytosol fractions | Amaranthus cruentus | - |
additional information | no enzyme activity is observed experimentally in chloroplasts of mesophyll and bundle sheath cells | Amaranthus cruentus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Amaranthus cruentus | pyruvate + CO2 + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD-malate dehydrogenase | - |
Amaranthus cruentus |
NAD-malic enzyme | - |
Amaranthus cruentus |
NAD-ME | - |
Amaranthus cruentus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Amaranthus cruentus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.6 | assay at | Amaranthus cruentus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Amaranthus cruentus | |
NADH | - |
Amaranthus cruentus |
Organism | Comment | Expression |
---|---|---|
Amaranthus cruentus | drought induces two of three isozymes in mesophyll and bundle sheath cells | up |