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Literature summary for 1.1.1.39 extracted from
- Reverse reaction of malic enzyme for HCO3- fixation into pyruvic acid to synthesize L-malic acid with enzymatic coenzyme regeneration (2008), Biosci. Biotechnol. Biochem., 72, 1278-1282.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is useful for production of L-malic acid with NADH generation including the reverse reaction of malic enzyme and the activity of glucose-6-phosphate dehydrogenase, EC1.1.1.49, from Leuconostoc mesenteroides, overview | Brevundimonas diminuta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | method optimization of the reverse reaction of the malic enzyme for HCO3- fixation into pyruvic acid to produce L-malic acid with NADH generation including the activity of glucose-6-phosphate dehydrogenase, EC1.1.1.49, from Leuconostoc mesenteroides | Brevundimonas diminuta |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Brevundimonas diminuta |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Brevundimonas diminuta | - |
pyruvate + CO2 + NADH | - |
r | |
| (S)-malate + NAD+ | Brevundimonas diminuta IFO 13182 | - |
pyruvate + CO2 + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | - |
- |
- |
| Brevundimonas diminuta IFO 13182 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 44fold | Brevundimonas diminuta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.989 | - |
purified native enzyme, reverse reaction | Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Brevundimonas diminuta | pyruvate + CO2 + NADH | - |
r | |
| (S)-malate + NAD+ | - |
Brevundimonas diminuta IFO 13182 | pyruvate + CO2 + NADH | - |
r | |
| pyruvate + NAD+ + HCO3- | method optimization of the reverse reaction of the malic enzyme for HCO3- fixation into pyruvic acid to produce L-malic acid with NADH generation including the activity of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, from Leuconostoc mesenteroides | Brevundimonas diminuta | (S)-malate + NADH | - |
? | |
| pyruvate + NAD+ + HCO3- | method optimization of the reverse reaction of the malic enzyme for HCO3- fixation into pyruvic acid to produce L-malic acid with NADH generation including the activity of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, from Leuconostoc mesenteroides | Brevundimonas diminuta IFO 13182 | (S)-malate + NADH | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at, reverse reaction | Brevundimonas diminuta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
reverse reaction | Brevundimonas diminuta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Brevundimonas diminuta | |
| NADH | - |
Brevundimonas diminuta | |
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Release 2023.1 (January 2023)
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