Crystallization (Comment) | Organism |
---|---|
quarternary complex of purified enzyme with NADH, tartronate, and Mg2+, hanging drop vapour diffusion method, from 100 mM Tris-SO4, pH 7.3, 100 mM sodium acetate, 15% PEG 4000, 5 mM NADH, 10 mM tartronate, 20 mM MgSO4, 10 mM 2-mercaptoethanol, and 0.02% sodium azide, for cryoprotection the crystals are soaked in 25% PEG 4000, 15 mM NAD+, 10 mM 2-mercaptoethanol, and 100 mM Tris-SO4, pH 7.5, for 2 h, then 24 h in the crystallization buffer plus 20% EG v/v, X-ray diffraction structure determination and analysis at 2.0 A resolution, structure modeling | Ascaris suum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tartronate | binding site structure at the active site, competitive | Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ascaris suum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ascaris suum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ | reaction mechanism, active site structure, enzyme-cofactor interactions, overview | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Ascaris suum | pyruvate + CO2 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ME | - |
Ascaris suum |
NAD-malic enzyme | - |
Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | binding site structure of NADH | Ascaris suum | |
NAD+ | conformational change upon binding | Ascaris suum |