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Literature summary for 1.1.1.39 extracted from

  • Coleman, D.E.; Rao, G.S.; Goldsmith, E.J.; Cook, P.F.; Harris, B.G.
    Crystal structure of the malic enzyme from Ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 A resolution (2002), Biochemistry, 41, 6928-6938.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in binary complex with NAD+, by hanging drop vapour diffusion method, from solution containing 5 mM NAD+, 10 mM tartronate, and 20 mM MgSO4, for cryoprotection soaking overnight in 100 mM Tris-HCl, pH 7.5, with 25% PEG w/v 15 mM NAD+, 10 mM 2-mercaptoethanol, and glycerol up to a final concentration of 20% v/v, X-ray diffraction structure determination and analysis at 2.3 A resolution, structure modeling Ascaris suum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Ascaris suum 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ascaris suum the Ascaris suum enzyme forms complexes with the structurally similar human enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
several steps Ascaris suum

Reaction

Reaction Comment Organism Reaction ID
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ active site structure, catalytic residues are Y126, R181, K199, D295, N343, and N479 Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the Ascaris suum enzyme forms complexes with the structurally similar human enzyme Ascaris suum ?
-
?

Subunits

Subunits Comment Organism
tetramer dimer of dimers Ascaris suum

Synonyms

Synonyms Comment Organism
NAD-malic enzyme
-
Ascaris suum

Cofactor

Cofactor Comment Organism Structure
NAD+ binding site structure Ascaris suum