Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Metallosphaera sedula |
expression in Escherichia coli | Hydrogenobaculum sp. Y04AAS1 |
expression in Escherichia coli | Ignisphaera aggregans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NADH | Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Ignisphaera aggregans | |
additional information | - |
NADPH | Km-value for NADPH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Ignisphaera aggregans | |
0.024 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Metallosphaera sedula | |
0.031 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Metallosphaera sedula | |
0.039 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Hydrogenobaculum sp. Y04AAS1 | |
0.046 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Hydrogenobaculum sp. Y04AAS1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobaculum sp. Y04AAS1 | - |
- |
- |
Ignisphaera aggregans | - |
- |
- |
Metallosphaera sedula | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
- |
Hydrogenobaculum sp. Y04AAS1 |
- |
Ignisphaera aggregans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Metallosphaera sedula | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Hydrogenobaculum sp. Y04AAS1 | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Ignisphaera aggregans | (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Metallosphaera sedula | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? | |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Hydrogenobaculum sp. Y04AAS1 | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? | |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate | Ignisphaera aggregans | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Ignisphaera aggregans | |
0.03 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Ignisphaera aggregans | |
0.06 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Metallosphaera sedula | |
0.07 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Metallosphaera sedula | |
0.12 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Hydrogenobaculum sp. Y04AAS1 | |
0.12 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Hydrogenobaculum sp. Y04AAS1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Metallosphaera sedula |
7 | - |
assay at | Hydrogenobaculum sp. Y04AAS1 |
7 | - |
assay at | Ignisphaera aggregans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme can use both NADH and NADPH with similar efficiency | Metallosphaera sedula | |
NADH | the enzyme can use both NADH and NADPH with similar efficiency | Hydrogenobaculum sp. Y04AAS1 | |
NADH | the enzyme can use both NADH and NADPH with similar efficiency | Ignisphaera aggregans | |
NADPH | the enzyme can use both NADH and NADPH with similar efficiency | Metallosphaera sedula | |
NADPH | the enzyme can use both NADH and NADPH with similar efficiency | Hydrogenobaculum sp. Y04AAS1 | |
NADPH | the enzyme can use both NADH and NADPH with similar efficiency | Ignisphaera aggregans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NADH | kcat/Km-value for NADH is above 20/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Ignisphaera aggregans | |
additional information | - |
NADPH | kcat/Km-value for NADPH is above 25/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Ignisphaera aggregans | |
2.1 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Metallosphaera sedula | |
2.5 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Metallosphaera sedula | |
2.7 | - |
NADPH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Hydrogenobaculum sp. Y04AAS1 | |
3.2 | - |
NADH | pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Hydrogenobaculum sp. Y04AAS1 |