Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-aspartate | - |
Escherichia coli W |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.025 mM, 70% activity lost after 5 min | Escherichia coli W | |
CoA | - |
Escherichia coli W | |
Cu2+ | 0.025 mM, 70% activity lost after 5 min | Escherichia coli W | |
D-malate | competitive inhibition | Escherichia coli W | |
malonate | competitive inhibition | Escherichia coli W | |
oxaloacetate | 50% inhibition with 0.09 mM | Escherichia coli W | |
p-(chloromercuri)benzoate | 0.0025 mM, 80% inhibition within 2 min | Escherichia coli W |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
NAD+ | - |
Escherichia coli W | |
0.32 | - |
Mn2+ | - |
Escherichia coli W | |
0.4 | - |
L-malate | - |
Escherichia coli W | |
2.3 | - |
NADP+ | - |
Escherichia coli W | |
5 | - |
oxaloacetate | - |
Escherichia coli W |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Escherichia coli W | |
Mg2+ | - |
Escherichia coli W | |
Mn2+ | - |
Escherichia coli W | |
Ni2+ | requirement for divalent cation | Escherichia coli W | |
Zn2+ | requirement for divalent cation | Escherichia coli W |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
198000 | - |
low speed sedimentation equilibrium | Escherichia coli W |
203000 | - |
sucrose density gradient centrifugation | Escherichia coli W |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Escherichia coli W | - |
CO2 + pyruvate + NADH | - |
? | |
Oxaloacetate | Escherichia coli W | - |
CO2 + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli W | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme is reversibly inhibited by air oxidation, activity is completely restored in 30 min by the addition of 2 mM dithiotreitol | Escherichia coli W |
Purification (Comment) | Organism |
---|---|
streptomycin, ammonium sulfate, heat treatment, acid treatment, calcium phosphate gel, DEAE-sephadex, hydroxylapatite, Sepharose 6B | Escherichia coli W |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
177 | - |
- |
Escherichia coli W |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 10 mM 2-mercaptoethanol, 10 mM MgCl2, 5 mM L-aspartate, 2 months, 10% activity lost | Escherichia coli W |
4°C, 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 10 mM 2-mercaptoethanol, 2 months, 15% activity lost | Escherichia coli W |
4°C, 50 mM Tris-HCl, pH 7.4, 1 month, 90% activity lost | Escherichia coli W |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Escherichia coli W | CO2 + pyruvate + NADH | - |
? | |
(S)-malate + NAD+ | - |
Escherichia coli W | CO2 + pyruvate + NADH | - |
r | |
(S)-malate + NADP+ | - |
Escherichia coli W | CO2 + pyruvate + NADPH | - |
? | |
Oxaloacetate | - |
Escherichia coli W | CO2 + pyruvate | - |
ir | |
Oxaloacetate | NAD+ inhibits oxaloacetate decarboxylation activity | Escherichia coli W | CO2 + pyruvate | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
with increasing substrate concentrations pH optimum is shifted towards higher pH values | Escherichia coli W |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli W | |
NADP+ | 2% activity | Escherichia coli W |