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Literature summary for 1.1.1.38 extracted from

  • Park, S-H.; Harris, B.G.; Cook, P.F.
    pH Dependence of kinetic parameters for oxalacetate decarboxylation and pyruvate reduction reactions catalyzed by malic enzyme (1986), Biochemistry, 25, 3752-3759.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Ascaris suum
-
CO2 + pyruvate + NADH
-
r
Oxaloacetate Ascaris suum
-
CO2 + pyruvate
-
ir
pyruvate + NADH + H+ Ascaris suum
-
L-lactate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35
-
-
Ascaris suum

Storage Stability

Storage Stability Organism
-20°C, 15 mM triethanolamine-maleate, pH 7.5, 1 mM EDTA, 10 mM dithiotreitol, 5% glycerol, several months, no loss of activity Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Ascaris suum CO2 + pyruvate + NADH
-
r
Oxaloacetate
-
Ascaris suum CO2 + pyruvate
-
ir
Oxaloacetate Mg2+ or Mn2+ are required Ascaris suum CO2 + pyruvate
-
ir
pyruvate + NADH + H+
-
Ascaris suum (S)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Ascaris suum L-lactate + NAD+
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
pyruvate in the presence of NAD+, 20 mM Mn2+ Ascaris suum
0.09
-
pyruvate in the absence of NAD+, 20 mM Mn2+ Ascaris suum
28
-
oxaloacetate in the absence of NAD+, 20 mM Mn2+ Ascaris suum
30
-
oxaloacetate in the presence of NAD+, 20 mM Mn2+ Ascaris suum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ascaris suum