Literature summary for 1.1.1.37 extracted from

Gharib, G.; Rashid, N.; Bashir, Q.; Gardner, Q.T.; Akhtar, M.; Imanaka, T.
Pcal_1699, an extremely thermostable malate dehydrogenase from hyperthermophilic archaeon Pyrobaculum calidifontis (2016), Extremophiles, 20, 57-67.

Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrobaculum calidifontis

Inhibitors

Inhibitors	Comment	Organism	Structure
guanidine hydrochloride	in the presence of 4 M guanidine hydrochloride enzyme structure is unfolded with complete loss of enzyme activity	Pyrobaculum calidifontis
additional information	urea cannot induce complete unfolding and inactivation of Pcal_1699 even at a final concentration of 8 M. Not inhibitory: EDTA Triton X-100, TWeen-20, dithiothreitol, glycerol	Pyrobaculum calidifontis
sodium dodecylsulfate	0.1%, 70% loss of activity, 1%, complete loss of activity	Pyrobaculum calidifontis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	oxaloacetate	pH 10, 55°C	Pyrobaculum calidifontis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	enzyme is not dependent on any metal ions	Pyrobaculum calidifontis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33292	-	4 * 33292, calculated, 4 * 35086, MALDI-TOF	Pyrobaculum calidifontis
35086	-	4 * 33292, calculated, 4 * 35086, MALDI-TOF	Pyrobaculum calidifontis
140000	-	gel filtration	Pyrobaculum calidifontis

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum calidifontis	A3MWU9	-	-
Pyrobaculum calidifontis JCM 11548	A3MWU9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
oxaloacetate + NADH + H+	-	Pyrobaculum calidifontis	(S)-malate + NAD+	-	?
oxaloacetate + NADH + H+	-	Pyrobaculum calidifontis JCM 11548	(S)-malate + NAD+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 33292, calculated, 4 * 35086, MALDI-TOF	Pyrobaculum calidifontis

Synonyms

Synonyms	Comment	Organism
Pcal_1699	-	Pyrobaculum calidifontis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Pyrobaculum calidifontis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
98	-	more than 90% residual activity after 6 h in boiling water. No significant change in CD spectra up to 100°C	Pyrobaculum calidifontis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
77	-	oxaloacetate	pH 10, 55°C	Pyrobaculum calidifontis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	carbonate-bicarbonate buffer	Pyrobaculum calidifontis

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no activity with NADPH	Pyrobaculum calidifontis
NADH	-	Pyrobaculum calidifontis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pyrobaculum calidifontis	calculated	-	6.9

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Release 2023.1 (January 2023)