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Literature summary for 1.1.1.37 extracted from

  • Madern, D.; Pfister, C.; Zaccai, G.
    Mutation at a single acidic amino acid enhances the halophilic behaviour of malate dehydrogenase from Haloarcula marismortui in physiological salts (1995), Eur. J. Biochem., 230, 1088-1095.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Haloarcula marismortui

Protein Variants

Protein Variants Comment Organism
E243R mutation does not affect enzyme activity. The mutant enzyme is more halophilic than the wild-type protein. It is more sensitive to temperature and requires significantly higher concentrations of NaCl or KCl for equivalent stability Haloarcula marismortui

General Stability

General Stability Organism
in KCl at 25°C, 50% residual activity is observed after 24 h for mutant enzyme E243R in 2.3 M salt, compared to 1.3 M salt for wild-type enzyme. In KCl at 4°C, the mutant shows 50% stability in 1.1 M salt Haloarcula marismortui

Organism

Organism UniProt Comment Textmining
Haloarcula marismortui Q07841
-
-
Haloarcula marismortui DSM 3752 Q07841
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloarcula marismortui

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Haloarcula marismortui (S)-malate + NAD+
-
?
oxaloacetate + NADH + H+
-
Haloarcula marismortui DSM 3752 (S)-malate + NAD+
-
?

Synonyms

Synonyms Comment Organism
MalDH
-
Haloarcula marismortui

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Haloarcula marismortui

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
t1/2 is 510 min for the wild-type enzyme and 15 min for mutant enzyme E243R Haloarcula marismortui

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Haloarcula marismortui

Cofactor

Cofactor Comment Organism Structure
NADH
-
Haloarcula marismortui