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Literature summary for 1.1.1.37 extracted from

  • Wang, Z.; Wang, B.; Ge, Y.; Pan, W.; Wang, J.; Xu, L.; Liu, A.; Zhu, G.
    Expression and identification of a thermostable malate dehydrogenase from multicellular prokaryote Streptomyces avermitilis MA-4680 (2011), Mol. Biol. Rep., 38, 1629-1636.
    View publication on PubMed

Application

Application Comment Organism
biotechnology MDH is widely used in coenzyme regeneration, antigen immunoassays and bioreactors Streptomyces avermitilis

Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons, recombinant expression of His-tagged MDH in Escherichia coli strain Rosetta (DE3) Streptomyces avermitilis

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Streptomyces avermitilis
Mn2+
-
Streptomyces avermitilis
Ni2+
-
Streptomyces avermitilis
oxaloacetate substrate inhibition Streptomyces avermitilis
Zn2+
-
Streptomyces avermitilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Streptomyces avermitilis
Cu2+ activates Streptomyces avermitilis
Mg2+ activates Streptomyces avermitilis
additional information Co2+ has no effect on the activity Streptomyces avermitilis
Na+ activates Streptomyces avermitilis
NH4+ activates Streptomyces avermitilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34689
-
x * 35000, SDS-PAGE, x * 34689, sequence calculation Streptomyces avermitilis
35000
-
x * 35000, SDS-PAGE, x * 34689, sequence calculation Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Streptomyces avermitilis
-
oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ Streptomyces avermitilis MA-4680
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
-
-
-
Streptomyces avermitilis MA-4680
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Streptomyces avermitilis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ 1246fold preference for oxaloacetate reduction over L-malate oxidation Streptomyces avermitilis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Streptomyces avermitilis MA-4680 oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ 1246fold preference for oxaloacetate reduction over L-malate oxidation Streptomyces avermitilis MA-4680 oxaloacetate + NADH + H+
-
r
additional information the recombinant SaMDH may also use NADPH as a cofactor although it is a highly NAD(H)-specific enzyme. No activity with malate and NADP+ as substrates Streptomyces avermitilis ?
-
?
additional information the recombinant SaMDH may also use NADPH as a cofactor although it is a highly NAD(H)-specific enzyme. No activity with malate and NADP+ as substrates Streptomyces avermitilis MA-4680 ?
-
?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE, x * 34689, sequence calculation Streptomyces avermitilis

Synonyms

Synonyms Comment Organism
MDH
-
Streptomyces avermitilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
recombinant enzyme Streptomyces avermitilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range, profile overview Streptomyces avermitilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant enzyme, half-life is 160 min, recombinant enzyme Streptomyces avermitilis
55
-
purified recombinant enzyme, stable below 55°C, loss of 18% activity after 10 min Streptomyces avermitilis
60
-
purified recombinant enzyme, loss of 47% activity after 10 min Streptomyces avermitilis
65
-
purified recombinant enzyme, loss of 80% activity after 10 min Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme Streptomyces avermitilis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 activity range Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
additional information the recombinant SaMDH may also use NADPH as a cofactor although it is a highly NAD(H)-specific enzyme, no activity with malate and NADP+ as substrates Streptomyces avermitilis
NAD+
-
Streptomyces avermitilis
NADH
-
Streptomyces avermitilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.27
-
oxaloacetate pH 8.0, 42°C Streptomyces avermitilis