Cloned (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | product inhibitor | Archaeoglobus fulgidus | |
NADH | substrate inhibition | Archaeoglobus fulgidus | |
oxaloacetate | substrate inhibition | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADH | pH 8.0, 65°C, at 0.4 mM oxaloacetate | Archaeoglobus fulgidus | |
0.043 | - |
oxaloacetate | pH 8.0, 65°C, at 0.4 mM NADH | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Archaeoglobus fulgidus |
70000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O08349 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.64 | - |
pH 8.0, 65°C, cell extract | Archaeoglobus fulgidus |
4799 | - |
pH 8.0, 65°C, purified enzyme | Archaeoglobus fulgidus |
Storage Stability | Organism |
---|---|
4°C, stable for several months under oxic conditions | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | the reduction of oxaloacetate with NADH is preferred. Malate is oxidized by NAD+ at 10% of the maximal velocity for the reduction of oxaloacetate with NADH. No oxidation of malate with NADP+ as coenzyme | Archaeoglobus fulgidus | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | the reduction of oxaloacetate with NADH is preferred. Malate is oxidized by NAD+ at 10% of the maximal velocity for the reduction of oxaloacetate with NADH. Maximal activity with NADPH is 10% compared to the activity with NADH | Archaeoglobus fulgidus | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Archaeoglobus fulgidus |
90 | - |
- |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 85 | between 50°C and 85°C the activity increases following the Arrhenius equation with a calculated activation energy of 61.6 kJ/mol | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
5 h, stable | Archaeoglobus fulgidus |
101 | - |
half-life: more than 80 min. Marked stabilizing effect of 1 M K2HPO4 with an increase in thermostability of approximately 40% at 101°C | Archaeoglobus fulgidus |
102 | - |
half-life: 30 min | Archaeoglobus fulgidus |
103 | - |
half-life: 18 min | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.2 | in both 50 mM Tricine-KOH and 50 mM potassium phosphate buffer | Archaeoglobus fulgidus |
8 | - |
assay at | Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no oxidation of malate with NADP+ as coenzyme | Archaeoglobus fulgidus | |
NADH | maximal activity with NADPH is 10% compared to the activity with NADH | Archaeoglobus fulgidus | |
NADPH | maximal activity with NADPH is 10% compared to the activity with NADH | Archaeoglobus fulgidus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
(S)-malate | pH 8.0, 65°C | Archaeoglobus fulgidus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Archaeoglobus fulgidus | isoelectric focusing, pH 310 | - |
4.5 |