Protein Variants | Comment | Organism |
---|---|---|
additional information | adsorption of the enzyme in 30 nm diameter Au nanoparticles for synthesis of bioconjugates, MDH:Au stoichiometry and kinetic parameters determined for MDH:Au, citrate synthase CS:Au, and three types of dual-activity MDH/CS:Au bioconjugates, method development, overview. The number of enzyme molecules adsorbed per particle is dependent upon the enzyme concentration in solution, with multilayers forming at high enzyme:Au solution ratios. The specific activity of adsorbed enzyme increases with increasing number adsorbed per particle for CS:Au, but is less sensitive to stoichiometry for MDH:Au | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxaloacetate | pH 8.1, 22°C | Sus scrofa | |
0.3 | - |
(S)-malate | pH 8.1, 22°C | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Sus scrofa | - |
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
182 | - |
pH 8.1, 22°C, specific activity of free and conjugated enzymes prepared at 300:1 solution enzyme:Au ratio | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malate dehydrogenase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature, oxidation and reduction | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
(S)-malate | pH 8.1, 22°C | Sus scrofa | |
377 | - |
oxaloacetate | pH 8.1, 22°C | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at, oxidation and reduction | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa | |
NADH | - |
Sus scrofa |