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Literature summary for 1.1.1.37 extracted from

  • Yao, Y.X.; Li, M.; Zhai, H.; You, C.X.; Hao, Y.J.
    Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis (2011), J. Plant Physiol., 168, 474-480.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, real-time quantitative RT-PCR expression analysis, recombinant ectopic expression in Solanum lycopersicum Malus domestica
gene MdcyMDH, DNA and amino acid sequence determination and analysis, genetic organization, expression analysis, overview. MdcyMDH gene, driven by theCaMV35S promoter, is introduced into tomato to evaluate the ex planta function of MdcyMDH ectopic expression on fruit acidity. MdcyMDH transcripts are detected in both fruits and functional leaves Malus domestica

Protein Variants

Protein Variants Comment Organism
additional information MdcyMDH overexpression contributed to malate accumulation in the apple callus and tomato Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
(S)-malate pH 7.5, 30°C Malus domestica
0.19
-
(S)-malate pH 7.9, 25°C Malus domestica
0.405
-
oxaloacetate pH 7.8, 30°C Malus domestica
1.4
-
NADH pH 7.8, 30°C Malus domestica
3.32
-
NAD+ pH 7.5, 30°C Malus domestica
3.32
-
NAD+ pH 7.9, 25°C Malus domestica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Malus domestica 5737
-
cytosol cytosolic malate dehydrogenase isozyme Malus domestica 5829
-
additional information subcellular localization analysis, overview Malus domestica
-
-
plasma membrane
-
Malus domestica 5886
-
plasma membrane cytosolic malate dehydrogenase isozyme Malus domestica 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Malus domestica
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Malus domestica A3DSX0
-
-
Malus domestica A3DSX0 gene MdcyMDH
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit expression level of MdcyMDH is positively correlated with MDH activity throughout fruit development, but not with malate content, especially in the ripening apple fruit Malus domestica
-
fruit enzyme expression throughout fruit development Malus domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Malus domestica oxaloacetate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
cyMDH
-
Malus domestica
cytosolic malate dehydrogenase
-
Malus domestica
cytosolic NAD-dependent malate dehydrogenase
-
Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, malate oxidation reaction Malus domestica
30
-
assay at Malus domestica
30
-
assay at, oxaloacetate reduction reaction Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.54
-
NAD+ pH 7.5, 30°C Malus domestica
3.21
-
NAD+ pH 7.9, 25°C Malus domestica
8.4
-
(S)-malate pH 7.9, 25°C Malus domestica
9.91
-
(S)-malate pH 7.5, 30°C Malus domestica
609.5
-
NADH pH 7.8, 30°C Malus domestica
4553
-
oxaloacetate pH 7.8, 30°C Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at, malate oxidation Malus domestica
7.8
-
assay at, oxaloacetate reduction Malus domestica
7.8
-
assay at, oxaloacetate reduction reaction Malus domestica
7.9
-
assay at, malate oxidation reaction Malus domestica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Malus domestica
NADH
-
Malus domestica

General Information

General Information Comment Organism
physiological function cyMDH is an enzyme crucial for malate synthesis in the cytosol. Involvement of MdcyMDH directly in malate synthesis and indirectly in malate accumulation through the regulation of genes/enzymes associated with malate degradation and transportation, gluconeogenesis and the tricarboxylic acid cycle. Malus domestica
physiological function cytosolic NAD-dependent malate dehydrogenase is an enzyme crucial for malate synthesis in the cytosol Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.765
-
NAD+ pH 7.9, 25°C Malus domestica
0.77
-
NAD+ pH 7.5, 30°C Malus domestica
44.26
-
(S)-malate pH 7.5, 30°C Malus domestica
44.26
-
(S)-malate pH 7.9, 25°C Malus domestica
435.4
-
NADH pH 7.8, 30°C Malus domestica
11240
-
oxaloacetate pH 7.8, 30°C Malus domestica