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Literature summary for 1.1.1.37 extracted from

  • Matsuda, T.; Takahashi-Yanaga, F.; Yoshihara, T.; Maenaka, K.; Watanabe, Y.; Miwa, Y.; Morimoto, S.; Kubohara, Y.; Hirata, M.; Sasaguri, T.
    Dictyostelium differentiation-inducing factor-1 binds to mitochondrial malate dehydrogenase and inhibits its activity (2010), J. Pharmacol. Sci., 112, 320-326.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one i.e. differentiation-inducing factor DIF-1, a morphogen secreted from Dictyostelium discoideum, inhibits proliferation of several cancer cells via suppression of the Wnt/beta -catenin signaling pathway, specifically to mMDH and inhibits its activity, no binding of cytosolic MDH, overview Homo sapiens
2-methoxy-1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one i.e. differentiation-inducing factor 2-MIDIF-1, a morphogen secreted from Dictyostelium discoideum, inhibits proliferation of several cancer cells via suppression of the Wnt/beta -catenin signaling pathway, binds specifically to mMDH and inhibits its activity, no binding of cytosolic MDH, overview Homo sapiens
additional information differentiation-inducing factors DIF-3, i.e. 1-(3-chloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one, and 6-MIDIF-3, a 6-methoxy isomer of DIF-3, bind spefically to mMDH, but do not inhibit the enzyme activity, no binding of cytosolic MDH Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic MDH isozyme Homo sapiens 5829
-
mitochondrion isozyme mMDH Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Homo sapiens
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Homo sapiens oxaloacetate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
cytosolic MDH
-
Homo sapiens
mitochondrial malate dehydrogenase
-
Homo sapiens
mMDH
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens

General Information

General Information Comment Organism
physiological function MDH is an essential enzyme in the tricarboxylic acid cycle. Inhibition of mMDH activity affects cell energy production, probably leading to the inhibition of proliferation. Inhibition of mMDH activity by DIF-1 and 2-MIDIF-1 can be one of the mechanisms to induce anti-proliferative effects, independent of the inhibition of the Wnt/beta-catenin signaling pathway Homo sapiens