Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one | i.e. differentiation-inducing factor DIF-1, a morphogen secreted from Dictyostelium discoideum, inhibits proliferation of several cancer cells via suppression of the Wnt/beta -catenin signaling pathway, specifically to mMDH and inhibits its activity, no binding of cytosolic MDH, overview | Homo sapiens | |
2-methoxy-1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one | i.e. differentiation-inducing factor 2-MIDIF-1, a morphogen secreted from Dictyostelium discoideum, inhibits proliferation of several cancer cells via suppression of the Wnt/beta -catenin signaling pathway, binds specifically to mMDH and inhibits its activity, no binding of cytosolic MDH, overview | Homo sapiens | |
additional information | differentiation-inducing factors DIF-3, i.e. 1-(3-chloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one, and 6-MIDIF-3, a 6-methoxy isomer of DIF-3, bind spefically to mMDH, but do not inhibit the enzyme activity, no binding of cytosolic MDH | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic MDH isozyme | Homo sapiens | 5829 | - |
mitochondrion | isozyme mMDH | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Homo sapiens | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Homo sapiens | oxaloacetate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cytosolic MDH | - |
Homo sapiens |
mitochondrial malate dehydrogenase | - |
Homo sapiens |
mMDH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | MDH is an essential enzyme in the tricarboxylic acid cycle. Inhibition of mMDH activity affects cell energy production, probably leading to the inhibition of proliferation. Inhibition of mMDH activity by DIF-1 and 2-MIDIF-1 can be one of the mechanisms to induce anti-proliferative effects, independent of the inhibition of the Wnt/beta-catenin signaling pathway | Homo sapiens |