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Literature summary for 1.1.1.37 extracted from

  • Fujii, T.; Oikawa, T.; Muraoka, I.; Soda, K.; Hata, Y.
    Crystallization and preliminary X-ray diffraction studies of tetrameric malate dehydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1 (2007), Acta Crystallogr. Sect. F, 63, 983-986.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
tetrameric enzyme, hanging drop vapour diffusion method, 15°C, 17 mg/ml protein in 10 mM potassium phosphate buffer, pH 7.0, equilibration of the 0.002 ml protein drop against 500 ml reservoir solution consisting of 1.4 M ammonium sulfate, 5% v/v MPD, 2 mM NAD+, 50 mM sodium citrate buffer, pH 5.5, 2-3 days, X-ray diffraction structure determination and analysis at 1.8 A resolution Flavobacterium frigidimaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Flavobacterium frigidimaris malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ Flavobacterium frigidimaris KUC-1 malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Flavobacterium frigidimaris Q25QU7
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-
Flavobacterium frigidimaris KUC-1 Q25QU7
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Flavobacterium frigidimaris oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle Flavobacterium frigidimaris oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Flavobacterium frigidimaris KUC-1 oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle Flavobacterium frigidimaris KUC-1 oxaloacetate + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer
-
Flavobacterium frigidimaris

Synonyms

Synonyms Comment Organism
MDH
-
Flavobacterium frigidimaris

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Flavobacterium frigidimaris
NADH dependent on Flavobacterium frigidimaris