KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADH | pH 8.0, 25°C, tetrameric enzyme form | Rhodopseudomonas palustris | |
0.022 | - |
oxaloacetate | pH 8.0, 25°C, tetrameric enzyme form | Rhodopseudomonas palustris | |
0.023 | - |
NADH | pH 8.0, 25°C, dimeric enzyme form | Rhodopseudomonas palustris | |
0.03 | - |
oxaloacetate | pH 8.0, 25°C, dimeric enzyme form | Rhodopseudomonas palustris | |
0.085 | - |
NAD+ | pH 9.0, 25°C, dimeric enzyme form | Rhodopseudomonas palustris | |
0.27 | - |
NAD+ | pH 9.0, 25°C, tetrameric enzyme form | Rhodopseudomonas palustris | |
0.53 | - |
(S)-malate | pH 9.0, 25°C, dimeric enzyme form | Rhodopseudomonas palustris | |
0.555 | - |
(S)-malate | pH 9.0, 25°C, tetrameric enzyme form | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, or tetramer, SDS-PAGE | Rhodopseudomonas palustris |
47000 | - |
or dimer, 4 * 47000, SDS-PAGE | Rhodopseudomonas palustris |
90000 | - |
dimeric enzyme form, gel filtration | Rhodopseudomonas palustris |
180000 | - |
tetrameric enzyme form, gel filtration | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodopseudomonas palustris | the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle | ? | - |
? | |
additional information | Rhodopseudomonas palustris f8pt | the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
Rhodopseudomonas palustris f8pt | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.55 | - |
tetrameric enzyme form | Rhodopseudomonas palustris |
1.61 | - |
dimeric enzyme form | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle | Rhodopseudomonas palustris | ? | - |
? | |
additional information | the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle | Rhodopseudomonas palustris f8pt | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Rhodopseudomonas palustris | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Rhodopseudomonas palustris f8pt | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, or tetramer, SDS-PAGE | Rhodopseudomonas palustris |
tetramer | or dimer, 4 * 47000, SDS-PAGE | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
reduction of oxaloacetate, dimeric enzyme form | Rhodopseudomonas palustris |
9 | - |
reduction of oxaloacetate, tetrameric enzyme form | Rhodopseudomonas palustris |
9.7 | - |
oxidation of malate, tetrameric enzyme form | Rhodopseudomonas palustris |
10.2 | - |
oxidation of malate, dimeric enzyme form | Rhodopseudomonas palustris |