Application | Comment | Organism |
---|---|---|
analysis | use of the thermostable enzyme from Vulcanithermus medioatlanticu DSM 14978 opens broad possibilities for the application of malate dehydrogenase as an analytical reagent and creation on biosensors for biochemical and cliniucal tests | Vulcanithermus mediatlanticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0266 | - |
NADH | pH 7.8, 25°C | Macromonas bipunctata | |
0.042 | - |
NADH | pH 7.8, 25°C | Vulcanithermus mediatlanticus | |
0.0476 | - |
oxaloacetate | pH 7.8, 25°C | Vulcanithermus mediatlanticus | |
0.053 | - |
oxaloacetate | pH 7.8, 25°C | Macromonas bipunctata | |
0.263 | - |
NAD+ | pH 9.0, 25°C | Macromonas bipunctata | |
0.4 | - |
NAD+ | pH 9.0, 25°C | Vulcanithermus mediatlanticus | |
0.645 | - |
(S)-malate | pH 9.0, 25°C | Macromonas bipunctata | |
1.67 | - |
(S)-malate | pH 9.0, 25°C | Vulcanithermus mediatlanticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Macromonas bipunctata |
58000 | - |
2 * 58000, SDS-PAGE | Vulcanithermus mediatlanticus |
92000 | - |
gel filtration | Macromonas bipunctata |
128000 | - |
gel filtration | Vulcanithermus mediatlanticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macromonas bipunctata | - |
- |
- |
Macromonas bipunctata D-405 | - |
- |
- |
Vulcanithermus mediatlanticus | - |
DSM 14978 | - |
Purification (Comment) | Organism |
---|---|
- |
Macromonas bipunctata |
- |
Vulcanithermus mediatlanticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Vulcanithermus mediatlanticus |
26.9 | - |
- |
Macromonas bipunctata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Macromonas bipunctata | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Vulcanithermus mediatlanticus | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Macromonas bipunctata D-405 | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Macromonas bipunctata |
dimer | 2 * 58000, SDS-PAGE | Vulcanithermus mediatlanticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Macromonas bipunctata |
82 | - |
- |
Vulcanithermus mediatlanticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
complete inactivation | Macromonas bipunctata |
50 | - |
15 min, stable | Vulcanithermus mediatlanticus |
60 | - |
15 min, about 25% loss of activity | Vulcanithermus mediatlanticus |
70 | - |
15 min, about 50% loss of activity | Vulcanithermus mediatlanticus |
80 | - |
15 min, about 70% loss of activity | Vulcanithermus mediatlanticus |
85 | - |
1 min, complete loss of activity | Vulcanithermus mediatlanticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
reduction of oxaloacetate | Vulcanithermus mediatlanticus |
7.7 | - |
reduction of oxaloacetate | Macromonas bipunctata |
8.5 | - |
oxidation of malate | Vulcanithermus mediatlanticus |
10.2 | - |
oxidation of malate | Macromonas bipunctata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Macromonas bipunctata | |
NAD+ | - |
Vulcanithermus mediatlanticus | |
NADH | - |
Macromonas bipunctata | |
NADH | - |
Vulcanithermus mediatlanticus |