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Literature summary for 1.1.1.37 extracted from

  • Cuevas, I.C.; Podesta, F.E.
    Purification and physical and kinetic characterization of an NAD+-dependent malate dehydrogenase from leaves of pineapple (Ananas comosus) (2000), Physiol. Plant., 108, 240-248.
No PubMed abstract available
Search for more literature for 1.1.1.37

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Mesembryanthemum crystallinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0152
-
 NADH pH 7.5 Ananas comosus
0.041
-
 NADH pH 6.8 Ananas comosus
0.0643
-
 oxaloacetate pH 6.8 Ananas comosus
0.22
-
 NAD+ pH 7.5 Ananas comosus
0.28
-
 NAD+ pH 6.8 Ananas comosus
0.287
-
 oxaloacetate pH 7.5 Ananas comosus
0.46
-
 L-malate pH 7.5 Ananas comosus
2.1
-
 L-malate pH 6.8 Ananas comosus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Zea mays 9507
-
chloroplast
-
 Arabidopsis thaliana 9507
-
cytoplasm
-
 Echinococcus granulosus 5737
-
cytosol
-
 Arabidopsis thaliana 5829
-
cytosol
-
 Echinococcus granulosus 5829
-
cytosol
-
 Mesembryanthemum crystallinum 5829
-
cytosol
-
 Ananas comosus 5829
-
cytosol
-
 Bryophyllum calycinum 5829
-
glyoxysome
-
 Ananas comosus 9514
-
glyoxysome
-
 Citrullus lanatus 9514
-
mitochondrion
-
 Arabidopsis thaliana 5739
-
mitochondrion
-
 Ananas comosus 5739
-
mitochondrion
-
 Citrullus lanatus 5739
-
mitochondrion
-
 Bryophyllum calycinum 5739
-
peroxisome
-
 Ananas comosus 5777
-
peroxisome
-
 Bryophyllum calycinum 5777
-
plastid
-
 Arabidopsis thaliana 9536
-
plastid
-
 Opuntia ficus-indica 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 2 * 39500, pH 7.0, SDS-PAGE Ananas comosus
39500
-
 4 * 39500, pH 8.0, SDS-PAGE Ananas comosus
160000
-
 native PAGE Ananas comosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Zea mays
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Arabidopsis thaliana
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Echinococcus granulosus
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Mesembryanthemum crystallinum
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Ananas comosus
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Citrullus lanatus
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Opuntia ficus-indica
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Bryophyllum calycinum
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
 pineapple, cv. Havaiano
-
Arabidopsis thaliana
-
-
-
Bryophyllum calycinum
-
-
-
Citrullus lanatus
-
 watermelon
-
Echinococcus granulosus
-
-
-
Mesembryanthemum crystallinum
-
-
-
Opuntia ficus-indica
-
 cactus
-
Zea mays
-
 corn
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Citrullus lanatus
-
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Ananas comosus
-
plant
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1494
-
 cytoplasmic isoenzyme Ananas comosus
2000 2500 mitochondrial isoenzyme Ananas comosus

Storage Stability

Storage Stability Organism
-20°C, activity tends to decay rapidly after purification Ananas comosus
-80°C, remains active for several weeks Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Arabidopsis thaliana oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Echinococcus granulosus oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Mesembryanthemum crystallinum oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Ananas comosus oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Citrullus lanatus oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Opuntia ficus-indica oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Bryophyllum calycinum oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Arabidopsis thaliana oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Echinococcus granulosus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Mesembryanthemum crystallinum oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Ananas comosus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Citrullus lanatus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Opuntia ficus-indica oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Bryophyllum calycinum oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH
-
 Zea mays L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Arabidopsis thaliana L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Echinococcus granulosus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Mesembryanthemum crystallinum L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Ananas comosus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Citrullus lanatus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Opuntia ficus-indica L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Bryophyllum calycinum L-malate + NAD+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 39500, pH 7.0, SDS-PAGE Ananas comosus
tetramer 4 * 39500, pH 8.0, SDS-PAGE Ananas comosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 activity increases up to Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7 oxaloacetate reduction Ananas comosus
6.8 7
-
 Ananas comosus

Cofactor

Cofactor Comment Organism Structure
NAD+ no detectable activity with NADP+ Ananas comosus