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Literature summary for 1.1.1.37 extracted from
- Purification and properties of malate dehydrogenase from the thermophilic archaebacterium Thermoplasma acidophilum (1986), Biol. Chem. Hoppe-Seyler, 367, 457-463.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0056 | - |
oxaloacetate | - |
Thermoplasma acidophilum |  |
| 0.0117 | - |
NADH | - |
Thermoplasma acidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36600 | - |
4 * 36600, SDS-PAGE | Thermoplasma acidophilum |
| 105000 | - |
gel filtration | Thermoplasma acidophilum |
| 130000 | - |
sedimentation equilibrium experiment | Thermoplasma acidophilum |
| 144000 | - |
cross-linking data | Thermoplasma acidophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malate + NAD+ | Bacillus subtilis | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Halobacterium salinarum | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Sulfolobus acidocaldarius | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Thermoplasma acidophilum | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Bacillus sp. (in: Bacteria) | - |
oxaloacetate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Methanospirillum hungatei | - |
- |
- |
| Sulfolobus acidocaldarius | - |
- |
- |
| Thermoplasma acidophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 180 | - |
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Halobacterium salinarum | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Sulfolobus acidocaldarius | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Thermoplasma acidophilum | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Bacillus sp. (in: Bacteria) | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Halobacterium salinarum | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Sulfolobus acidocaldarius | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Thermoplasma acidophilum | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Bacillus sp. (in: Bacteria) | oxaloacetate + NADH + H+ | - |
r | |
| oxaloacetate + NADH | - |
Bacillus subtilis | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Halobacterium salinarum | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Sulfolobus acidocaldarius | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Thermoplasma acidophilum | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Bacillus sp. (in: Bacteria) | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Methanospirillum hungatei | L-malate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Bacillus subtilis |
| tetramer | - |
Sulfolobus acidocaldarius |
| tetramer | - |
Bacillus sp. (in: Bacteria) |
| tetramer | 4 * 36600, SDS-PAGE | Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermoplasma acidophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
quite heat-stable, stable up to, loses 50% of its original activity after 10 min at 77°C | Thermoplasma acidophilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
oxaloacetate | - |
Thermoplasma acidophilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
reduction of oxaloacetate | Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sulfolobus acidocaldarius | |
| NAD+ | purified enzyme shows activity with both coenzymes | Thermoplasma acidophilum | |
| NADP+ | - |
Sulfolobus acidocaldarius | |
| NADP+ | purified enzyme shows activity with both coenzymes | Thermoplasma acidophilum | |
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