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Literature summary for 1.1.1.37 extracted from

  • Ohshima, T.; Sakuraba, H.
    Purification and characterization of malate dehydrogenase from the phototrophic bacterium, Rhodopseudomonas capsulata (1986), Biochim. Biophys. Acta, 869, 171-177.
No PubMed abstract available
Search for more literature for 1.1.1.37

General Stability

General Stability Organism
addition of NAD+, NADH or oxaloacetate stabilizes the enzyme Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 NADH
-
 Rhodobacter capsulatus
0.048
-
 NAD+
-
 Rhodobacter capsulatus
0.085
-
 oxaloacetate
-
 Rhodobacter capsulatus
2.1
-
 L-malate
-
 Rhodobacter capsulatus
2.2
-
 Ketomalonate
-
 Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Rhodobacter capsulatus
80000
-
 gel filtration Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Thermus aquaticus
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Cereibacter sphaeroides
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Rhodobacter capsulatus
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Rhodobacter capsulatus
-
 strain 37b, strain number DSM 938
-
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides
-
 Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
348
-
-
 Rhodobacter capsulatus

Storage Stability

Storage Stability Organism
-10°C, in 20 mM potassium phosphate buffer, pH 7.0, 0.01% 2-mercaptoethanol/40% glycerol, can be stored for at least 2 months without loss of activity Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Thermus aquaticus oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Cereibacter sphaeroides oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Rhodobacter capsulatus oxaloacetate + NADH + H+
-
 r
2-oxobutyrate + NADH
-
 Rhodobacter capsulatus 2-hydroxybutyrate + NAD+
-
 ?
alpha-keto-malonate + NADH
-
 Rhodobacter capsulatus ? + NAD+
-
 ?
malate + NAD+
-
 Thermus aquaticus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Cereibacter sphaeroides oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Rhodobacter capsulatus oxaloacetate + NADH + H+
-
 r
meso-tartrate + NAD+ slightly oxidized, 0.82 of the rate for L-malate oxidation Rhodobacter capsulatus ? + NADH
-
 r
additional information D-malate, L-tartarate, D-tartarate, hydroxymalonate, dihydroxyfumarate, L-lactate, D-lactate Dl-alpha-hydroxybutyrate, pyruvate, alpha-hydroxypyruvate, alpha-ketoisovalerate and alpha-ketoglutarate are no substrates Rhodobacter capsulatus ?
-
 ?
oxaloacetate + NADH
-
 Thermus aquaticus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Cereibacter sphaeroides L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Rhodobacter capsulatus L-malate + NAD+
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Thermus aquaticus
dimer 2 * 35000, SDS-PAGE Rhodobacter capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 loses its activity completely upon incubation for 10 min Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3 7 reduction of ketomalonate Rhodobacter capsulatus
7.75 8.5 reduction of oxaloacetate Rhodobacter capsulatus
9.5
-
 L-malate oxidation Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
NAD+ can not be replaced by NADP+ Rhodobacter capsulatus