Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Mycolicibacterium phlei | |
cysteine | - |
Mycolicibacterium phlei | |
glutathione | - |
Mycolicibacterium phlei |
Crystallization (Comment) | Organism |
---|---|
- |
Mycolicibacterium phlei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus subtilis | |
Co2+ | - |
Mycolicibacterium phlei | |
Cu2+ | - |
Mycolicibacterium phlei | |
Hg2+ | - |
Mycolicibacterium phlei | |
iodoacetate | - |
Mycolicibacterium phlei | |
Mg2+ | - |
Bacillus subtilis | |
Mg2+ | - |
Mycolicibacterium phlei | |
Mn2+ | - |
Mycolicibacterium phlei | |
Ni2+ | - |
Mycolicibacterium phlei | |
p-chloromercuribenzoate | - |
Mycolicibacterium phlei | |
Zn2+ | - |
Bacillus subtilis | |
Zn2+ | - |
Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
NADH | - |
Bacillus subtilis | |
0.0286 | - |
NADH | - |
Mycolicibacterium phlei | |
0.0443 | - |
oxaloacetate | - |
Mycolicibacterium phlei | |
0.061 | - |
oxaloacetate | - |
Bacillus subtilis | |
0.14 | - |
NAD+ | - |
Bacillus subtilis | |
0.459 | - |
NAD+ | - |
Mycolicibacterium phlei | |
0.833 | - |
L-malate | - |
Mycolicibacterium phlei | |
0.9 | - |
L-malate | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21550 | - |
4 * 21550, SDS-PAGE | Mycolicibacterium phlei |
86200 | - |
SDS-PAGE | Mycolicibacterium phlei |
86860 | - |
gel filtration | Mycolicibacterium phlei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | Bacillus subtilis | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Escherichia coli | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Homo sapiens | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Bos taurus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Neurospora crassa | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Mycolicibacterium phlei | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Mycobacterium tuberculosis | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Mycobacterium scrofulaceum | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Mycobacterium kansasii | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bos taurus | - |
bovine | - |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
human | - |
Mycobacterium kansasii | - |
- |
- |
Mycobacterium scrofulaceum | - |
- |
- |
Mycobacterium tuberculosis | - |
H37Rv, H37Ra, BCG | - |
Mycolicibacterium phlei | - |
- |
- |
Neurospora crassa | - |
- |
- |
no activity in Mycobacterium fortuitum | - |
- |
- |
no activity in Mycobacterium smegmatis | - |
- |
- |
no activity in Mycobacterium smegmatis 607 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium phlei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
heart | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43.47 | - |
- |
Mycolicibacterium phlei |
Storage Stability | Organism |
---|---|
20°C, dilute enzyme preparation stable for at least 2 weeks | Mycolicibacterium phlei |
25°C, loses not more than 20% activity within 2 weeks | Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Homo sapiens | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Neurospora crassa | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Mycolicibacterium phlei | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Mycobacterium tuberculosis | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Mycobacterium scrofulaceum | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Mycobacterium kansasii | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Homo sapiens | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Neurospora crassa | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Mycolicibacterium phlei | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Mycobacterium tuberculosis | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Mycobacterium scrofulaceum | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Mycobacterium kansasii | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | - |
Bacillus subtilis | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Escherichia coli | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Homo sapiens | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Bos taurus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Neurospora crassa | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Mycolicibacterium phlei | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Mycobacterium tuberculosis | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Mycobacterium scrofulaceum | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Mycobacterium kansasii | L-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Bacillus subtilis |
tetramer | - |
Escherichia coli |
tetramer | 4 * 21550, SDS-PAGE | Mycolicibacterium phlei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
50 | - |
- |
Escherichia coli |
50 | - |
thermostable up to for 15 min | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
maximum conversion of malate to oxalacetate | Mycolicibacterium phlei |
9.2 | 9.6 | reduction of oxalacetate to malate | Mycolicibacterium phlei |
9.5 | - |
- |
Mycolicibacterium phlei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Mycolicibacterium phlei |