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Literature summary for 1.1.1.37 extracted from

  • Tyagi, A.K.; Siddiqui, F.A.; Venkitasubramanian, T.A.
    Studies on the purification and characterization of malate dehydrogenase from Mycobacterium phlei (1977), Biochim. Biophys. Acta, 485, 255-267.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Mycolicibacterium phlei
cysteine
-
Mycolicibacterium phlei
glutathione
-
Mycolicibacterium phlei

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Mycolicibacterium phlei

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Bacillus subtilis
Co2+
-
Mycolicibacterium phlei
Cu2+
-
Mycolicibacterium phlei
Hg2+
-
Mycolicibacterium phlei
iodoacetate
-
Mycolicibacterium phlei
Mg2+
-
Bacillus subtilis
Mg2+
-
Mycolicibacterium phlei
Mn2+
-
Mycolicibacterium phlei
Ni2+
-
Mycolicibacterium phlei
p-chloromercuribenzoate
-
Mycolicibacterium phlei
Zn2+
-
Bacillus subtilis
Zn2+
-
Mycolicibacterium phlei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
NADH
-
Bacillus subtilis
0.0286
-
NADH
-
Mycolicibacterium phlei
0.0443
-
oxaloacetate
-
Mycolicibacterium phlei
0.061
-
oxaloacetate
-
Bacillus subtilis
0.14
-
NAD+
-
Bacillus subtilis
0.459
-
NAD+
-
Mycolicibacterium phlei
0.833
-
L-malate
-
Mycolicibacterium phlei
0.9
-
L-malate
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21550
-
4 * 21550, SDS-PAGE Mycolicibacterium phlei
86200
-
SDS-PAGE Mycolicibacterium phlei
86860
-
gel filtration Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Bacillus subtilis
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Escherichia coli
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Homo sapiens
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Bos taurus
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Neurospora crassa
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Mycolicibacterium phlei
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Mycobacterium tuberculosis
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Mycobacterium scrofulaceum
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Mycobacterium kansasii
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bos taurus
-
bovine
-
Escherichia coli
-
-
-
Homo sapiens
-
human
-
Mycobacterium kansasii
-
-
-
Mycobacterium scrofulaceum
-
-
-
Mycobacterium tuberculosis
-
H37Rv, H37Ra, BCG
-
Mycolicibacterium phlei
-
-
-
Neurospora crassa
-
-
-
no activity in Mycobacterium fortuitum
-
-
-
no activity in Mycobacterium smegmatis
-
-
-
no activity in Mycobacterium smegmatis 607
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycolicibacterium phlei

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-
heart
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.47
-
-
Mycolicibacterium phlei

Storage Stability

Storage Stability Organism
20°C, dilute enzyme preparation stable for at least 2 weeks Mycolicibacterium phlei
25°C, loses not more than 20% activity within 2 weeks Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Homo sapiens oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Bos taurus oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Neurospora crassa oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Mycolicibacterium phlei oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Mycobacterium tuberculosis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Mycobacterium scrofulaceum oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Mycobacterium kansasii oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Homo sapiens oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Bos taurus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Neurospora crassa oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Mycolicibacterium phlei oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Mycobacterium tuberculosis oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Mycobacterium scrofulaceum oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Mycobacterium kansasii oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH
-
Bacillus subtilis L-malate + NAD+
-
r
oxaloacetate + NADH
-
Escherichia coli L-malate + NAD+
-
r
oxaloacetate + NADH
-
Homo sapiens L-malate + NAD+
-
r
oxaloacetate + NADH
-
Bos taurus L-malate + NAD+
-
r
oxaloacetate + NADH
-
Neurospora crassa L-malate + NAD+
-
r
oxaloacetate + NADH
-
Mycolicibacterium phlei L-malate + NAD+
-
r
oxaloacetate + NADH
-
Mycobacterium tuberculosis L-malate + NAD+
-
r
oxaloacetate + NADH
-
Mycobacterium scrofulaceum L-malate + NAD+
-
r
oxaloacetate + NADH
-
Mycobacterium kansasii L-malate + NAD+
-
r

Subunits

Subunits Comment Organism
tetramer
-
Bacillus subtilis
tetramer
-
Escherichia coli
tetramer 4 * 21550, SDS-PAGE Mycolicibacterium phlei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
Bacillus subtilis
50
-
-
Escherichia coli
50
-
thermostable up to for 15 min Mycolicibacterium phlei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
maximum conversion of malate to oxalacetate Mycolicibacterium phlei
9.2 9.6 reduction of oxalacetate to malate Mycolicibacterium phlei
9.5
-
-
Mycolicibacterium phlei

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
Mycolicibacterium phlei