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Literature summary for 1.1.1.365 extracted from

  • Wagschal, K.; Jordan, D.B.; Hart-Cooper, W.M.; Chan, V.J.
    Penicillium camemberti galacturonate reductase C-1 oxidation/reduction of uronic acids and substrate inhibition mitigation by aldonic acids (2020), Int. J. Biol. Macromol., 153, 1090-1098 .
    View publication on PubMed
Search for more literature for 1.1.1.365

Inhibitors

Inhibitors Comment Organism Structure
C-4 epimer galacturonate displays marked substrate inhibition Penicillium camemberti
glucuronate weak substrate inhibition Penicillium camemberti
L-gulonate competitive inhibitor, Ki in the low mM range Penicillium camemberti
L-gulono-1,4-lactone competitive inhibitor, Ki in the low mM range Penicillium camemberti
additional information substrate inhibition by aldonic acids. The products L-galactonate and L-galactono-1,4-lactone are observed to mitigate substrate inhibition by galacturonate, with the lactone having a greater effect than the acid Penicillium camemberti

Organism

Organism UniProt Comment Textmining
Penicillium camemberti
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucurono-3,6-lactone + NADPH + H+
-
 Penicillium camemberti L-gulono-1,4-lactone
-
 r
L-galactonate + NADP+
-
 Penicillium camemberti D-galacturonate + NADPH + H+
-
 r
L-gluconate + NADP+
-
 Penicillium camemberti D-glucuronate + NADPH + H+
-
 r
additional information the enzyme catalyzes the reduction of the C-1 carbon of D-glucuronate and C-4 epimer D-galacturonate to their corresponding aldonic acids. It is active on both glucuronic acid and galacturonic acid, with similar substrate specificities using the preferred cosubstrate NADPH. Substrate acceptance extends to lactone congeners, and D-glucurono-3,6-lactone is converted to L-gulono-1,4-lactone Penicillium camemberti ?
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-

Synonyms

Synonyms Comment Organism
galacturonate reductase
-
 Penicillium camemberti
PcGOR
-
 Penicillium camemberti

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Penicillium camemberti
NADPH
-
 Penicillium camemberti

General Information

General Information Comment Organism
physiological function Penicillium camemberti reduces the C-1 carbon of D-glucuronate and C-4 epimer D-galacturonate to their corresponding aldonic acids, important reactions in both pectin catabolism and ascorbate biosynthesis. Enzyme PcGOR is active on both glucuronic acid and galacturonic acid, with similar substrate specificities (kcat/Km) using the preferred cosubstrate NADPH. Substrate acceptance extends to lactone congeners, and D-glucurono-3,6-lactone is converted to L-gulono-1,4-lactone, an immediate precursor of ascorbate Penicillium camemberti