Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADP+ | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADP+ | at pH 8.0 and 30°C | Pseudomonas putida | |
0.127 | - |
NAD+ | at pH 8.0 and 30°C | Pseudomonas putida | |
0.946 | - |
D-glucose 6-phosphate | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
1.137 | - |
D-glucose 6-phosphate | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | Pseudomonas putida | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NAD+ | Pseudomonas putida KT 2240 | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | Pseudomonas putida | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | Pseudomonas putida KT 2240 | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.006 | - |
with NADP+ as cofactor, at pH 8.0 and 30°C | Pseudomonas putida |
0.025 | - |
with NAD+ as cofactor, at pH 8.0 and 30°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | - |
Pseudomonas putida | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NAD+ | - |
Pseudomonas putida KT 2240 | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Pseudomonas putida | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Pseudomonas putida KT 2240 | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH-1 | - |
Pseudomonas putida |
glucose-6-phosphate dehydrogenase | - |
Pseudomonas putida |
zwf-1 | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102 | - |
NADP+ | at pH 8.0 and 30°C | Pseudomonas putida | |
277 | - |
NAD+ | at pH 8.0 and 30°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADP+ | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.111 | - |
NADP+ | at pH 8.0 and 30°C | Pseudomonas putida | |
1.148 | - |
NAD+ | at pH 8.0 and 30°C | Pseudomonas putida |