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Literature summary for 1.1.1.363 extracted from
- Site-directed mutagenesis to facilitate X-ray structural studies of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase (1993), Protein Sci., 2, 859-662.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop method, crystallization of six cysteine-containing mutants (Q56C, S61C, S132C, S215C, Q365C, S428C) of the enzyme and the successful preparation and crystallization of a heavy atom derivative of mutant enzyme S215C | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | P11411 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NAD+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? |  |
| D-glucose 6-phosphate + NADP+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Leuconostoc mesenteroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Leuconostoc mesenteroides | |
| NADP+ | - |
Leuconostoc mesenteroides | |
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Release 2023.1 (January 2023)
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