Literature summary for 1.1.1.363 extracted from

Simons, J.R.; Mosisch, M.; Torda, A.E.; Hilterhaus, L.
Site directed immobilization of glucose-6-phosphate dehydrogenase via thiol-disulfide interchange: influence on catalytic activity of cysteines introduced at different positions (2013), J. Biotechnol., 167, 1-7.

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Protein Variants

Protein Variants	Comment	Organism
D205C	enzyme variant with cysteine close to the dimer interface, about 30% loss of specific activity compared to wild-type enzyme shows changes in activity and the efficacy of immobilization.	Leuconostoc mesenteroides
D453C	enzyme variant with cysteine far from the active center, no significant loss in specific activity compared to wild-type enzyme, in contrast to wild-type enzyme, the mutant enzyme is readily immobilited	Leuconostoc mesenteroides
L218C	enzyme variant with cysteine close to the active center, about 30% loss of specific activity compared to wild-type enzyme, the mutant enzyme shows almost complete immobilization but poor carrier activity	Leuconostoc mesenteroides

General Stability

General Stability	Organism
eluted mutant enzyme D453C shows almost double the activity of the immobilized enzyme, which is consistent with 49% activity loss due to immobilization. Mutant enzyme D205C produces a 1.8-fold higher activity compared to its immobilized state. Eluted mutant enzyme L218C shows 9.9 times the activity of its immobilized state	Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
69000	-	x * 69000, SDS-PAGE	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	-	-	-

Storage Stability

Storage Stability	Organism
4°C, 8 months, enzym variants L218C, D205C or D453C, no loss of activity	Leuconostoc mesenteroides

Subunits

Subunits	Comment	Organism
?	x * 69000, SDS-PAGE	Leuconostoc mesenteroides

Synonyms

Synonyms	Comment	Organism
G6PDH	-	Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Leuconostoc mesenteroides

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Release 2023.1 (January 2023)