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Literature summary for 1.1.1.363 extracted from

  • Olive, C.; Geroch, M.E.; Levy, H.R.
    Glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides. Kinetic studies (1971), J. Biol. Chem., 246, 2047-2057.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by iodoacetate, iodoacetamide, and p-hydroxymercuribenzoate Leuconostoc mesenteroides
NADH product inhibition. With NAD+ as the varied substrate and glucose 6-phosphate, nonsaturating, linear noncompetitive inhibition is observed. When an experiment is performed using saturating glucose 6-phosphate, the inhibition again appears to be noncompetitive. When glucose 6-phosphate is the varied substrate inhibition by NADH appears to be noncompetitive whether NAD+ is nonsaturating or saturating Leuconostoc mesenteroides
NADPH NADPH is tested as an alternate product inhibitor for the NAD+-linked reaction. With glucose 6-phosphate saturating and NAD+ varied the inhibition is linear noncompetitive Leuconostoc mesenteroides
pyridoxal 5'-phosphate inhibition in phosphate buffers at pH 7.7 and pH 6.3. 0.1 mM Pyridoxal 5’-phosphate produces 50% inhibition at pH 7.7. At the lower pH more pyridoxal 5’-phosphate is required to give the same degree of inhibition. The enzyme must be incubated with pyridoxal 5’-phosphate prior to assaying in order to achieve maximum inhibition. Pyridoxamine 5’-phosphate and pyridoxal are only slightly inhibitory. When glucose 6-phosphate is varied at a constant, nearly saturating concentration of NAD+ pyridoxal 5’-phosphate behaves like a competitive inhibitor. When NAD+ is varied and glucose 6-phosphate maintained at nearly saturating concentration, inhibition by pyridoxal 5’-phosphate is noncompetitive Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the Km-value of NAD+ is insensitive to ionic strength over the range tested but that the Km for glucose 6-phosphate is affected. This effect is greatest at pH 9.0 Leuconostoc mesenteroides
0.006
-
 NADP+ 25°C, pH 7.2, cosubstrate: D-glucose 6-phosphate Leuconostoc mesenteroides
0.011
-
 thionicotinamide-NAD+ 25°C, pH 7.2, cosubstrate: D-glucose 6-phosphate Leuconostoc mesenteroides
0.053
-
 D-glucose 6-phosphate 25°C, pH 7.8, cosubstrate: NAD+ Leuconostoc mesenteroides
0.081
-
 D-glucose 6-phosphate 25°C, pH 7.2, cosubstrate: NADP+ Leuconostoc mesenteroides
0.106
-
 NAD+ 25°C, pH 7.8, cosubstrate: D-glucose 6-phosphate Leuconostoc mesenteroides
10
-
 D-galactose 6-phosphate 25°C, pH 7.8, cosubstrate: NAD+ Leuconostoc mesenteroides
12
-
 2-deoxy-D-glucose 6-phosphate 25°C, pH 7.8, cosubstrate: NAD+ Leuconostoc mesenteroides
50
-
 D-glucose 6-sulfate 25°C, pH 7.8, cosubstrate: NAD+ Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NAD+ Leuconostoc mesenteroides
-
 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NADP+ Leuconostoc mesenteroides
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose 6-phosphate + NAD+ low activity Leuconostoc mesenteroides ?
-
 ?
D-galactose 6-phosphate + NAD+ low activity Leuconostoc mesenteroides ?
-
 ?
D-glucose 6-phosphate + NAD+
-
 Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NAD+ the mechanism with NAD+ is more complex compared to the reaction with D-glucose 6-phosphate and NADP+ Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+ ordered, sequential mechanism for this reaction in which NADP+ is bound first to the enzyme and NADPH released last Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose 6-phosphate + thionicotinamide-NAD+
-
 Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + thionicotinamide-NADH + H+
-
 ?
D-glucose 6-sulfate + NAD+ low activity Leuconostoc mesenteroides ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+ the mechanism with NAD+ is more complex compared to the reaction with D-glucose 6-phosphate and NADP+ Leuconostoc mesenteroides
NADP+ ordered, sequential mechanism for this reaction in which NADP+ is bound first to the enzyme and NADPH released last Leuconostoc mesenteroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.039
-
 pyridoxal 5'-phosphate 25°C, pH 7.7, competitive inhibition when glucose 6-phosphate is varied at a constant, nearly saturating concentration of NAD+ Leuconostoc mesenteroides