Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | in 4 M guanidine hydrochloride, the dimeric enzyme dissociates to subunits and is extensively unfolded | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
in 4 M guanidine-HCl, the dimeric enzyme glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides dissociates to subunits and is extensively unfolded. Rapid dilution of this high guanidine hydrochloride concentration allowes the enzyme to partially renature. The fraction of the enzyme which does not renature aggregates and precipitates out of solution, a process which can not be substantially prevented by stabilizing additives. A renaturation mechanism is described, which involves a bi-unimolecular (subunit association-folding) reaction sequence. This mechanism involves an inactive, dimeric, glucose-6-phosphate dehydrogenase-folding intermediate | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Leuconostoc mesenteroides |