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Literature summary for 1.1.1.363 extracted from

  • Levy, H.R.; Vought, V.E.; Yin, X.; Adams, M.J.
    Identification of an arginine residue in the dual coenzyme-specific glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides that plays a key role in binding NADP+ but not NAD+ (1996), Arch. Biochem. Biophys., 326, 145-151.
    View publication on PubMed
Search for more literature for 1.1.1.363

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain SU294 Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
R46A mutant glucose-6-phosphate dehydrogenases with coenzyme specificity that favors NAD+, whereas the wild-type enzyme prefers NADP+ as coenzyme Leuconostoc mesenteroides
R46A the enzyme's Km and Ki values for NADP+ are greatly increased (2-3 orders of magnitude) Leuconostoc mesenteroides
R46C mutant glucose-6-phosphate dehydrogenases with coenzyme specificity that favors NAD+, whereas the wild-type enzyme prefers NADP+ as coenzyme Leuconostoc mesenteroides
R46Q the enzyme's Km and Ki values for NADP+ are greatly increased (2-3 orders of magnitude) Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
NADP+
-
 Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00799
-
 NADP+ wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.0692
-
 D-glucose 6-phosphate wild type enzyme, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.089
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
0.0891
-
 D-glucose 6-phosphate mutant enzyme R46Q, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.114
-
 D-glucose 6-phosphate wild type enzyme, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.162
-
 NAD+ wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.175
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
0.175
-
 D-glucose 6-phosphate mutant enzyme R46A, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.233
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
0.233
-
 NAD+ mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.334
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
0.334
-
 D-glucose 6-phosphate mutant enzyme R46Q, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.467
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant Leuconostoc mesenteroides
0.467
-
 D-glucose 6-phosphate mutant enzyme R46A, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
0.854
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
0.854
-
 NADP+ mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1.05
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
1.05
-
 NAD+ mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
5.57
-
 NADP+ mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NAD(P)+
-
 Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+
-
 ?
D-glucose 6-phosphate + NAD+
-
 Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Leuconostoc mesenteroides

Synonyms

Synonyms Comment Organism
G6PD
-
 Leuconostoc mesenteroides
glucose-6-phosphate dehydrogenase
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Leuconostoc mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
522
-
 D-glucose 6-phosphate wild type enzyme, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
825
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
825
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
825
-
 D-glucose 6-phosphate mutant enzyme R46Q, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
887
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
887
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
887
-
 D-glucose 6-phosphate mutant enzyme R46Q, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1125
-
 D-glucose 6-phosphate wild type enzyme, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1535
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
1535
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
1535
-
 D-glucose 6-phosphate mutant enzyme R46A, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1800
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
1800
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
1800
-
 D-glucose 6-phosphate mutant enzyme R46A, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Leuconostoc mesenteroides

Cofactor

Cofactor Comment Organism Structure
NAD+ can utilize either NADP+ or NAD+ as coenzyme Leuconostoc mesenteroides
NAD+ wild-type enzyme prefers NADP+ as coenzyme Leuconostoc mesenteroides
NADP+ can utilize either NADP+ or NAD+ as coenzyme Leuconostoc mesenteroides
NADP+ wild-type enzyme prefers NADP+ as coenzyme. Arg46 plays a key role in NADP+-binding Leuconostoc mesenteroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00337
-
 NADP+ wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1.79
-
 NADP+ mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1.8
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
8.23
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
8.23
-
 NADP+ mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.57
-
 NADP+ mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
323
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
323
-
 NADP+ mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
1039
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
1462
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
1462
-
 NAD+ mutant enzyme R46A, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
2650
-
 D-glucose 6-phosphate mutant enzyme R46Q, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
2656
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
3533
-
 NAD+ mutant enzyme R46Q, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
3540
-
 NADP+ 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
3850
-
 D-glucose 6-phosphate mutant enzyme R46A, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
3854
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
4583
-
 D-glucose 6-phosphate wild type enzyme, with NADP+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
6950
-
 NAD+ wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
8767
-
 D-glucose 6-phosphate mutant enzyme R46A, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
8771
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46A Leuconostoc mesenteroides
9267
-
 D-glucose 6-phosphate mutant enzyme R46Q, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
9270
-
 D-glucose 6-phosphate 25°C, pH 7.6, mutant enzyme R46Q Leuconostoc mesenteroides
16250
-
 D-glucose 6-phosphate wild type enzyme, with NAD+ as cosubstrate, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides
65330
-
 NADP+ wild type enzyme, in 33 mM Tris-HCl, pH 7.6, at 25°C Leuconostoc mesenteroides