Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rickettsia felis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 10% (w/v) PEG 8000, 8% (w/v) ethylene glycol, 100 mM HEPES-NaOH pH 7.5 | Rickettsia felis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
gel filtration | Rickettsia felis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-CoA + NADPH + H+ | Rickettsia felis | - |
(R)-3-hydroxyacyl-CoA + NADP+ | - |
r | |
3-oxoacyl-CoA + NADPH + H+ | Rickettsia felis ATCC VR-1525 | - |
(R)-3-hydroxyacyl-CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rickettsia felis | Q4UN54 | - |
- |
Rickettsia felis ATCC VR-1525 | Q4UN54 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 75 gel filtration | Rickettsia felis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-CoA + NADPH + H+ | - |
Rickettsia felis | (R)-3-hydroxyacyl-CoA + NADP+ | - |
r | |
3-oxoacyl-CoA + NADPH + H+ | - |
Rickettsia felis ATCC VR-1525 | (R)-3-hydroxyacyl-CoA + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 26000, SDS-PAGE | Rickettsia felis |
homotetramer | 4 * 25700, calculated from amino acid sequence | Rickettsia felis |
Synonyms | Comment | Organism |
---|---|---|
AcAc-CoA reductase | - |
Rickettsia felis |
PhbB | - |
Rickettsia felis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rickettsia felis |