Cloned (Comment) | Organism |
---|---|
gene fabG1, DNA sequence determination and analysis, expression in the fabG1-deficient mutant, phylogenetic tree, sequence comparison of FabG1 to FabG6 | Haloarcula hispanica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption or knockout of fabG1 abolishes HA synthesis, and complementation of the DELTAfabG1 mutant with the fabG1 gene restores both PHA synthesis capability and the NADPH-dependent acetoacetyl-CoA reductase activity. Heterologous coexpression of the PHA synthase genes, phaEC together with fabG1, but not its five paralogs, reconstructs the PHA biosynthetic pathway in Haloferax volcanii, a PHA-defective haloarchaeon | Haloarcula hispanica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | Haloarcula hispanica | FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | Haloarcula hispanica DSM 4426 | FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula hispanica | G0HY85 | or ATCC 33960, gene fabG1 | - |
Haloarcula hispanica DSM 4426 | G0HY85 | or ATCC 33960, gene fabG1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | - |
Haloarcula hispanica | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase | Haloarcula hispanica | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Haloarcula hispanica DSM 4426 | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | FabG1 exhibits NADPH-dependent activity of acetoacetyl-CoA reductase | Haloarcula hispanica DSM 4426 | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | FabG belongs to the short-chain dehydrogenase/reductase superfamily, whose members are homologous in sequence and have several conserved motifs | Haloarcula hispanica |
PHA-specific acetoacetyl-CoA reductase | - |
Haloarcula hispanica |
polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase | - |
Haloarcula hispanica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Haloarcula hispanica |