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Literature summary for 1.1.1.36 extracted from

  • Liu, Q.; Ouyang, S.P.; Chung, A.; Wu, Q.; Chen, G.Q.
    Microbial production of R-3-hydroxybutyric acid by recombinant E. coli harboring genes of phbA, phbB, and tesB (2007), Appl. Microbiol. Biotechnol., 76, 811-818.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene phbB, functional expression in Escherichia coli strains BW25113 and DH5alpha, co-expression with genes phbA and tesB, encoding beta-ketothiolase and thioesterase II, respectively, to reconstruct the R-3-hydroxybutyric acid production pathway, optimization of culture conditions, overview Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + NADPH + H+ Cupriavidus necator
-
3-oxobutyryl-CoA + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
gene phbB
-
no activity in Escherichia coli
-
strain DH5alpha
-
no activity in Escherichia coli DH5-alpha
-
strain DH5alpha
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53 0.62 recombinant enzyme in transformed Escherichia coli strain DH5alpha Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + NADPH + H+
-
Cupriavidus necator 3-oxobutyryl-CoA + NADP+
-
r

Synonyms

Synonyms Comment Organism
acetoacetyl CoA reductase
-
Cupriavidus necator
PhbB
-
Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Cupriavidus necator
NADPH
-
Cupriavidus necator