Cloned (Comment) | Organism |
---|---|
wild type (HsMFE-2) and its variants are expressed in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D370A | site-directed mutagenesis | Homo sapiens |
D490A | site-directed mutagenesis | Homo sapiens |
D510A | site-directed mutagenesis, inactive | Homo sapiens |
D517A | site-directed mutagenesis | Homo sapiens |
E366A | site-directed mutagenesis, kcat/Km 100times lower than that of the wild type | Homo sapiens |
E408A | site-directed mutagenesis | Homo sapiens |
G16S | site-directed mutagenesis | Homo sapiens |
H406A | site-directed mutagenesis | Homo sapiens |
H515A | site-directed mutagenesis | Homo sapiens |
H532A | site-directed mutagenesis | Homo sapiens |
additional information | constructs are tested for complementation in Saccharomyces cerevisiae | Homo sapiens |
Y347A | site-directed mutagenesis | Homo sapiens |
Y410A | site-directed mutagenesis | Homo sapiens |
Y505A | site-directed mutagenesis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
alignment of the amino acid sequence of the hydratase 2 domain from human MFE-2 with those of other known hydratase 2 domains from eukaryotes shown | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.023 | - |
strain HsMFE-2(Y347A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.023 | - |
strain HsMFE-2(Y410A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.039 | - |
strain HsMFE-2(E408A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.041 | - |
strain HsMFE-2(D490A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.046 | - |
strain HsMFE-2(H406A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.047 | - |
strain HsMFE-2(D370A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.048 | - |
strain HsMFE-2(D517A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.051 | - |
strain HsMFE-2(D510A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.056 | - |
strain HsMFE-2(E366A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.066 | - |
strain HsMFE-2(H532A), (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.082 | - |
strain HsMFE-2, (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
0.091 | - |
strain UTL-7A, (3R)-hydroxyacyl-CoA dehydrogenase | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
(3R)-hydroxyacyl-CoA dehydrogenase | domain in human MFE-2 | Homo sapiens |