Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM155 | Zoogloea ramigera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | above 0.01 mM | Zoogloea ramigera | |
propionylacetyl-CoA | - |
Zoogloea ramigera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
acetoacetyl-CoA | - |
Zoogloea ramigera | |
0.002 | - |
propionylacetyl-CoA | - |
Zoogloea ramigera | |
0.01 | - |
butyrylacetyl-CoA | - |
Zoogloea ramigera | |
0.02 | 0.021 | NADPH | cosubstrate acetoacetyl-CoA | Zoogloea ramigera | |
0.026 | - |
D-3-hydroxybutyryl-CoA | - |
Zoogloea ramigera | |
0.74 | - |
2-methylacetoacetyl-CoA | - |
Zoogloea ramigera | |
0.99 | - |
Acetoacetyl-S-(D-pantetheine)11-pivalate | - |
Zoogloea ramigera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Zoogloea ramigera | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
4 * 24000 SDS-PAGE | Zoogloea ramigera |
25200 | - |
4 * 25200 gene sequence | Zoogloea ramigera |
100000 | - |
gel filtration | Zoogloea ramigera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH | Zoogloea ramigera | biosynthesis of poly(D-3-hydroxybutyrate) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoogloea ramigera | - |
gene cloned in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose chromatography, Blue gel column | Zoogloea ramigera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
608 | - |
- |
Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylacetoacetyl-CoA + NADPH | - |
Zoogloea ramigera | 2-methyl-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH | biosynthesis of poly(D-3-hydroxybutyrate) | Zoogloea ramigera | ? | - |
? | |
acetoacetyl-CoA + NADPH + H+ | - |
Zoogloea ramigera | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-S-(D-pantetheine)11-pivalate + NADPH | - |
Zoogloea ramigera | 3-hydroxybutyryl-S-(D-pantetheine)11-pivalate + NADP+ | - |
? | |
butyrylacetyl-CoA + NADPH | - |
Zoogloea ramigera | 3-hydroxyhexanoyl-CoA + NADP+ | - |
? | |
propionylacetyl-CoA + NADPH | - |
Zoogloea ramigera | 3-hydroxypentanoyl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25200 gene sequence | Zoogloea ramigera |
tetramer | 4 * 24000 SDS-PAGE | Zoogloea ramigera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
butyrylacetyl-CoA | - |
Zoogloea ramigera | |
27 | - |
2-methylacetoacetyl-CoA | - |
Zoogloea ramigera | |
90 | - |
D-3-hydroxybutyryl-CoA | - |
Zoogloea ramigera | |
124 | - |
propionylacetyl-CoA | - |
Zoogloea ramigera | |
267 | - |
Acetoacetyl-S-(D-pantetheine)11-pivalate | - |
Zoogloea ramigera | |
300 | - |
acetoacetyl-CoA | - |
Zoogloea ramigera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Zoogloea ramigera | |
NADPH | B-specific, i.e. transfer of pro-S-hydrogen from NADPH to acetoacetyl-CoA | Zoogloea ramigera | |
NADPH | absolute specificity | Zoogloea ramigera |