General Stability | Organism |
---|---|
glycerol stabilizes during purification | Zoogloea ramigera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | markedly at concentrations above 0.005 mM and NADPH 0.01 mM, less inhibition when NADPH increases from 0.01 to 1 mM | Zoogloea ramigera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
4 * 25500, SDS-PAGE | Zoogloea ramigera |
92000 | - |
gel electrophoresis | Zoogloea ramigera |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | during purification | Zoogloea ramigera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pigeon | - |
- |
- |
Zoogloea ramigera | - |
I-16-M | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, DEAE-Sephasrose CL-6B, hydroxyapatite, Red-Sepharose CL-6B chromatography | Zoogloea ramigera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Zoogloea ramigera | - |
liver | - |
Pigeon | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
412 | - |
- |
Zoogloea ramigera |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris/HCl buffer, pH 7.5, 10 mM 2-mercaptoethanol, 50% glycerol, several months | Zoogloea ramigera |
0°C and -20°C, 10 mM Tris/HCl buffer, pH 7.5, 10 mM 2-mercaptoethanol, rather unstable | Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | - |
Zoogloea ramigera | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | - |
Pigeon | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
D-3-hydroxybutyryl-CoA + NADP+ | - |
Zoogloea ramigera | acetoacetyl-CoA + NADPH | - |
r | |
additional information | - |
Pigeon | ? | - |
? | |
additional information | poor substrate: D-3-hydroxyvaleryl-CoA | Zoogloea ramigera | ? | - |
? | |
additional information | not: L-3-hydroxyacyl-CoA | Zoogloea ramigera | ? | - |
? | |
additional information | not: D-3-hydroxyacyl-CoA's with chain length exceeding 6 | Zoogloea ramigera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25500, SDS-PAGE | Zoogloea ramigera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Zoogloea ramigera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Zoogloea ramigera | |
NADP+ | - |
Pigeon | |
NADPH | specific for | Zoogloea ramigera | |
NADPH | specific for | Pigeon |