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Literature summary for 1.1.1.357 extracted from

  • Fan, L.; Peng, G.; Hussain, A.; Fazli, L.; Guns, E.; Gleave, M.; Qi, J.
    The steroidogenic enzyme AKR1C3 regulates stability of the ubiquitin ligase Siah2 in prostate cancer cells (2015), J. Biol. Chem., 290, 20865-20879.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in mouse PCa cells and of FLAG-AKR1C3 in mouse Rv1 cells, recombinant expression of His-tagged AKR1C3 in Escherichia coli strain BL21 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P42330
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged AKR1C3 from Escherichia coli strain BL21 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
DU-145 cell
-
 Homo sapiens
-
LNCaP cell
-
 Homo sapiens
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PC-3 cell
-
 Homo sapiens
-
prostate cancer cell parallel expression of Siah2 and AKR1C3 in human prostate cancer tissues Homo sapiens
-

Synonyms

Synonyms Comment Organism
AKR1C3
-
 Homo sapiens
More cf. EC 1.1.1.239 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens R1881 treatment decreases AKR1C3 transcripts by about 1.5fold in Rv1 cells, AKR1C3 transcripts are androgen-repressed down

General Information

General Information Comment Organism
physiological function the steroidogenic enzyme AKR1C3 regulates stability of the ubiquitin ligase Siah2 in prostate cancer cells. AKR1C3 binds and stabilizes Siah2 by blocking Siah2 self-ubiquitination and degradation. It has a catalytic independent role on androgen receptor activity via Siah2 that promotes activity of androgen receptor in prostate cancer cells. AKR1C3 is a downstream effector of Siah2 in Rv1 cells Homo sapiens