Cloned (Comment) | Organism |
---|---|
recombinant expression in mouse PCa cells and of FLAG-AKR1C3 in mouse Rv1 cells, recombinant expression of His-tagged AKR1C3 in Escherichia coli strain BL21 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P42330 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AKR1C3 from Escherichia coli strain BL21 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
DU-145 cell | - |
Homo sapiens | - |
LNCaP cell | - |
Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
prostate cancer cell | parallel expression of Siah2 and AKR1C3 in human prostate cancer tissues | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
AKR1C3 | - |
Homo sapiens |
More | cf. EC 1.1.1.239 | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | R1881 treatment decreases AKR1C3 transcripts by about 1.5fold in Rv1 cells, AKR1C3 transcripts are androgen-repressed | down |
General Information | Comment | Organism |
---|---|---|
physiological function | the steroidogenic enzyme AKR1C3 regulates stability of the ubiquitin ligase Siah2 in prostate cancer cells. AKR1C3 binds and stabilizes Siah2 by blocking Siah2 self-ubiquitination and degradation. It has a catalytic independent role on androgen receptor activity via Siah2 that promotes activity of androgen receptor in prostate cancer cells. AKR1C3 is a downstream effector of Siah2 in Rv1 cells | Homo sapiens |