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Literature summary for 1.1.1.357 extracted from

  • Oppermann, U.C.; Maser, E.
    Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni (1996), Eur. J. Biochem., 241, 744-749.
    View publication on PubMed
Search for more literature for 1.1.1.357

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 Fusidic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
0.0068
-
 Fusidic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
0.0111
-
 5beta-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
0.0152
-
 3alpha-hydroxydesogestrel pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
0.0161
-
 5alpha-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
0.019
-
 5alpha-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
0.0201
-
 5beta-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
0.0207
-
 epiandrosterone pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
0.0216
-
 3alpha-hydroxydesogestrel pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
0.0244
-
 androsterone pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
0.025
-
 3-dehydro-alpha-ecdysone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
0.025
-
 cholic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
0.0267
-
 androsterone pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
0.028
-
 3-dehydro-alpha-ecdysone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
0.0301
-
 cholic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
0.0328
-
 androstandiol pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
0.035
-
 3-dehydro-beta-ecdysone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
0.0357
-
 androstandione pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
0.61
-
 Metyrapone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
0.63
-
 Metyrapone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
0.9
-
 4-Nitroacetophenone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1
-
 4-nitrobenzaldehyde pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1
-
 4-Nitroacetophenone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1
-
 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.09
-
 4-nitrobenzaldehyde pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
2.1
-
 metyrapol pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Comamonas testosteroni 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 SDS-PAGE Comamonas testosteroni
28000
-
 SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3alpha-hydroxysteroid + NAD(P)+ Comamonas testosteroni
-
 a 3-oxosteroid + NAD(P)H + H+
-
 ?
a 3alpha-hydroxysteroid + NAD(P)+ Pseudomonas sp.
-
 a 3-oxosteroid + NAD(P)H + H+
-
 ?
a 3alpha-hydroxysteroid + NAD(P)+ Comamonas testosteroni ATCC 11996
-
 a 3-oxosteroid + NAD(P)H + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni P80702
-
-
Comamonas testosteroni ATCC 11996 P80702
-
-
Pseudomonas sp. P80701 Boehringer patent strain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+ 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone i.e. NKI 42255 Comamonas testosteroni 2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+
-
 ?
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+ 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone i.e. NKI 42255 Comamonas testosteroni ATCC 11996 2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+
-
 ?
3-dehydro-alpha-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni alpha-ecdysone + NAD(P)+
-
 ?
3-dehydro-alpha-ecdysone + NAD(P)H + H+
-
 Pseudomonas sp. alpha-ecdysone + NAD(P)+
-
 ?
3-dehydro-alpha-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni ATCC 11996 alpha-ecdysone + NAD(P)+
-
 ?
3-dehydro-beta-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni beta-ecdysone + NAD(P)+
-
 ?
3-dehydro-beta-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni ATCC 11996 beta-ecdysone + NAD(P)+
-
 ?
3alpha-hydroxydesogestrel + NAD(P)+
-
 Comamonas testosteroni 3-oxodesogestrel + NAD(P)H + H+
-
 ?
3alpha-hydroxydesogestrel + NAD(P)+
-
 Pseudomonas sp. 3-oxodesogestrel + NAD(P)H + H+
-
 ?
4-nitroacetophenone + NAD(P)H + H+
-
 Comamonas testosteroni 1-(4-nitrophenyl)ethanol + NAD(P)+
-
 ?
4-nitroacetophenone + NAD(P)H + H+
-
 Pseudomonas sp. 1-(4-nitrophenyl)ethanol + NAD(P)+
-
 ?
4-nitrobenzaldehyde + NAD(P)H + H+
-
 Comamonas testosteroni (4-nitrophenyl)methanol + NAD(P)+
-
 ?
4-nitrobenzaldehyde + NAD(P)H + H+
-
 Pseudomonas sp. (4-nitrophenyl)methanol + NAD(P)+
-
 ?
5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Comamonas testosteroni (5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. (5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
5beta-dihydrotestosterone + NAD(P)H + H+
-
 Comamonas testosteroni (5beta,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
5beta-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. (5beta,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
a 3alpha-hydroxysteroid + NAD(P)+
-
 Comamonas testosteroni a 3-oxosteroid + NAD(P)H + H+
-
 ?
a 3alpha-hydroxysteroid + NAD(P)+
-
 Pseudomonas sp. a 3-oxosteroid + NAD(P)H + H+
-
 ?
a 3alpha-hydroxysteroid + NAD(P)+
-
 Comamonas testosteroni ATCC 11996 a 3-oxosteroid + NAD(P)H + H+
-
 ?
androstandiol + NAD(P)+
-
 Comamonas testosteroni (5alpha,17beta)-17-hydroxyandrostan-3-one + NAD(P)H + H+
-
 ?
androstandione + NAD(P)H + H+
-
 Comamonas testosteroni 3-hydroxyandrostan-17-one + NAD(P)+
-
 ?
androsterone + NAD(P)+
-
 Comamonas testosteroni (5alpha)-androstane-3,17-dione + NAD(P)H + H+
-
 ?
androsterone + NAD(P)+
-
 Pseudomonas sp. (5alpha)-androstane-3,17-dione + NAD(P)H + H+
-
 ?
cholic acid + NAD(P)+
-
 Comamonas testosteroni (5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+
-
 ?
cholic acid + NAD(P)+
-
 Pseudomonas sp. (5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+
-
 ?
epiandrosterone + NAD(P)+
-
 Comamonas testosteroni (5alpha)-androstane-3,17-dione + NAD(P)H + H+
-
 ?
fusidic acid + NAD(P)+
-
 Comamonas testosteroni (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+
-
 ?
fusidic acid + NAD(P)+
-
 Pseudomonas sp. (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+
-
 ?
metyrapol + NAD(P)+
-
 Comamonas testosteroni 2-methyl-1,2-di(pyridin-3-yl)propan-1-one + NAD(P)H + H+
-
 ?
metyrapone + NAD(P)H + H+
-
 Comamonas testosteroni 2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+
-
 ?
metyrapone + NAD(P)H + H+
-
 Pseudomonas sp. 2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+
-
 ?
additional information no activity with testosterone, 3-oxo-desogestrel, progesterone, 4-nitrobenzalcohol, trans-benzene dihydrodiol and cis-benzene dihydrodiol Comamonas testosteroni ?
-
 ?
additional information no activity with testosterone, 3-oxo-desogestrel, progesterone, NKI 42255, trans-benzene dihydrodiol and cis-benzene dihydrodiol Pseudomonas sp. ?
-
 ?
additional information no activity with testosterone, 3-oxo-desogestrel, progesterone, 4-nitrobenzalcohol, trans-benzene dihydrodiol and cis-benzene dihydrodiol Comamonas testosteroni ATCC 11996 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Comamonas testosteroni
monomer 1 * 28000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
HSD 28
-
 Comamonas testosteroni
HSD 29
-
 Pseudomonas sp.

General Information

General Information Comment Organism
metabolism enzyme is involved in inactivation of steroid hormones Comamonas testosteroni
metabolism enzyme is involved in inactivation of steroid hormones Pseudomonas sp.