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Literature summary for 1.1.1.356 extracted from

  • Alam, J.; Beyer, N.; Liu, H.W.
    Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC) (2004), Biochemistry, 43, 16450-16460.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Yersinia pseudotuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose pH 7.5, 37°C, cosubstrate: NADPH Yersinia pseudotuberculosis
0.139
-
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose pH 7.5, 37°C, cosubstrate: NADH Yersinia pseudotuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35234
-
2 * 35234, calculated from sequence Yersinia pseudotuberculosis
36000
-
2 * 36000, SDS-PAGE Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ Yersinia pseudotuberculosis the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides GDP-beta-L-colitose + NAD(P)+
-
?
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ Yersinia pseudotuberculosis VIA the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides GDP-beta-L-colitose + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis
-
-
-
Yersinia pseudotuberculosis VIA
-
-
-

Purification (Commentary)

Purification (Comment) Organism
N-terminal His6-tagged ColC Yersinia pseudotuberculosis

Storage Stability

Storage Stability Organism
-80°C, stable for at least 6 months Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides Yersinia pseudotuberculosis GDP-beta-L-colitose + NAD(P)+
-
?
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides Yersinia pseudotuberculosis VIA GDP-beta-L-colitose + NAD(P)+
-
?
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADH + H+ bifunctional enzyme catalyzing the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose Yersinia pseudotuberculosis GDP-beta-L-colitose + NAD+
-
?
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADH + H+ bifunctional enzyme catalyzing the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose Yersinia pseudotuberculosis VIA GDP-beta-L-colitose + NAD+
-
?
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADPH + H+ the enzyme catalyzes the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose. The stereochemical preference of ColC with regard to the transfer of one of the diastereotopic methylene hydrogens at C-4 of the dihydronicotinamide ring is established to be pro-S stereospecific Yersinia pseudotuberculosis GDP-beta-L-colitose + NADP+
-
?
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADPH + H+ the enzyme catalyzes the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose. The stereochemical preference of ColC with regard to the transfer of one of the diastereotopic methylene hydrogens at C-4 of the dihydronicotinamide ring is established to be pro-S stereospecific Yersinia pseudotuberculosis VIA GDP-beta-L-colitose + NADP+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 36000, SDS-PAGE Yersinia pseudotuberculosis
homodimer 2 * 35234, calculated from sequence Yersinia pseudotuberculosis

Synonyms

Synonyms Comment Organism
ColC
-
Yersinia pseudotuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Yersinia pseudotuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Yersinia pseudotuberculosis
NADPH
-
Yersinia pseudotuberculosis