Cloned (Comment) | Organism |
---|---|
- |
Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0426 | - |
5'-hydroxyaverantin | in 30 mM potassium phosphate buffer (pH 7.5), at 37°C | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25873 | - |
3 * 25873, calculated from amino acid sequence | Levilactobacillus brevis |
25918 | - |
3 * 25918 MALDI TOF mass spectrometry | Levilactobacillus brevis |
33000 | - |
3 * 33000, SDS-PAGE | Levilactobacillus brevis |
100000 | - |
gel filtration | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis | - |
5'-oxoaverantin + NADPH + H+ | - |
ir | |
5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis IFO 12005 | - |
5'-oxoaverantin + NADPH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Levilactobacillus brevis IFO 12005 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration | Levilactobacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00613 | - |
cell extract, at pH 7.5 and 37°C | Levilactobacillus brevis |
0.552 | - |
after 90fold purification, at pH 7.5 and 37°C | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-hydroxyaverantin + NADP+ | - |
Levilactobacillus brevis | 5'-oxoaverantin + NADPH + H+ | - |
ir | |
5'-hydroxyaverantin + NADP+ | - |
Levilactobacillus brevis IFO 12005 | 5'-oxoaverantin + NADPH + H+ | - |
ir | |
additional information | no activity with NAD+ | Levilactobacillus brevis | ? | - |
? | |
additional information | no activity with NAD+ | Levilactobacillus brevis IFO 12005 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 33000, SDS-PAGE | Levilactobacillus brevis |
homotrimer | 3 * 25873, calculated from amino acid sequence | Levilactobacillus brevis |
homotrimer | 3 * 25918 MALDI TOF mass spectrometry | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
alcohol dehydrogenase | - |
Levilactobacillus brevis |
HAVN dehydrogenase | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | - |
Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | no activity is detected above pH 9.0 | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Levilactobacillus brevis |