Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is used in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to (S)-3-hydroxybutyryl-CoA | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
gene paaH1, recombinant expression of C-terminally His6-tagged wild-type or selenomethionine-labeled enzymes in Escherichia coli strain B834 | Cupriavidus necator |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, mixing of 30 mg/ml wild-type protein or 25 mg/ml selenomethionine-labeled enzyme in 40 mM Tris-HCl, pH 8.0, 1 mM DTT, with reservoir solution containing 1.4 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.0, and 0.2 M sodium chloride, 22°C, 7 days, X-ray diffraction structure determination and analysis at 2.6 A resolution, single-wavelength anomalous dispersion method | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutanoyl-CoA + NAD+ | Cupriavidus necator | - |
acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutanoyl-CoA + NAD+ | Cupriavidus necator ATCC 17699 | - |
acetoacetyl-CoA + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0KEY8 | - |
- |
Cupriavidus necator ATCC 17699 | Q0KEY8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged wild-type or selenomethionine-labeled enzymes from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
Cupriavidus necator | acetoacetyl-CoA + NADH + H+ | - |
r | |
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
Cupriavidus necator ATCC 17699 | acetoacetyl-CoA + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
(S)-3-hydroxybutyryl-CoA dehydrogenase | - |
Cupriavidus necator |
PaaH1 | - |
Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cupriavidus necator | |
NADH | - |
Cupriavidus necator |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the second step in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA | Cupriavidus necator |