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Literature summary for 1.1.1.35 extracted from

  • Kim, E.; Kim, K.
    Cloning, expression, purification, crystallization and X-ray crystallographic analysis of (S)-3-hydroxybutyryl-CoA dehydrogenase from Clostridium butyricum (2014), Acta Crystallogr. Sect. F, 70, 485-488.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme in Escherichia coli strain B834 Clostridium butyricum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, mixing of 30 mg/ml protein in 40 mM Tris-HCl, pH 8.0, 1 mM DTT, with reservoir solution containing 2 M ammonium sulfate, 0.1 M CAPS, pH 10.5, and 0.2 M lithium sulfate, 22°C, 7 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method and structure modeling Clostridium butyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
-
Clostridium butyricum
60000
-
recombinant His6-tagged enzyme, gel filtration Clostridium butyricum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutanoyl-CoA + NAD+ Clostridium butyricum
-
acetoacetyl-CoA + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Clostridium butyricum C4IEM5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration Clostridium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutanoyl-CoA + NAD+
-
Clostridium butyricum acetoacetyl-CoA + NADH + H+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 30000, recombinant His6-tagged enzyme, SDS-PAGE Clostridium butyricum

Synonyms

Synonyms Comment Organism
(S)-3-hydroxybutyryl-CoA dehydrogenase
-
Clostridium butyricum
CbHBD
-
Clostridium butyricum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Clostridium butyricum
NADH
-
Clostridium butyricum

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the second step in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA Clostridium butyricum