Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged enzyme in Escherichia coli strain B834 | Clostridium butyricum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, mixing of 30 mg/ml protein in 40 mM Tris-HCl, pH 8.0, 1 mM DTT, with reservoir solution containing 2 M ammonium sulfate, 0.1 M CAPS, pH 10.5, and 0.2 M lithium sulfate, 22°C, 7 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method and structure modeling | Clostridium butyricum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
- |
Clostridium butyricum |
60000 | - |
recombinant His6-tagged enzyme, gel filtration | Clostridium butyricum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutanoyl-CoA + NAD+ | Clostridium butyricum | - |
acetoacetyl-CoA + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium butyricum | C4IEM5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration | Clostridium butyricum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxybutanoyl-CoA + NAD+ | - |
Clostridium butyricum | acetoacetyl-CoA + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 30000, recombinant His6-tagged enzyme, SDS-PAGE | Clostridium butyricum |
Synonyms | Comment | Organism |
---|---|---|
(S)-3-hydroxybutyryl-CoA dehydrogenase | - |
Clostridium butyricum |
CbHBD | - |
Clostridium butyricum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridium butyricum | |
NADH | - |
Clostridium butyricum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the second step in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA | Clostridium butyricum |