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Literature summary for 1.1.1.35 extracted from

  • He, X.Y.; Yang, S.Y.
    Molecular cloning, expression in Escherichia coli, and characterization of a novel L-3-hydroxyacyl coenzyme A dehydrogenase from pig liver (1998), Biochim. Biophys. Acta, 1392, 119-126.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
NADH
-
Sus scrofa
0.003
-
acetoacetyl-CoA
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 33000, SDS-PAGE Sus scrofa
66000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Sus scrofa
-
acetoacetyl-CoA + NADH
-
r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpressed in Escherichia coli, phosphocellulose Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
511
-
recombinant enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+
-
Sus scrofa acetoacetyl-CoA + NADH
-
r
3-ketohexadecanoyl-CoA + NADH
-
Sus scrofa (S)-3-hydroxhexadecanoyl-CoA + NAD+
-
r
3-ketooctanoyl-CoA + NADH
-
Sus scrofa (S)-3-hydroxyoctanoyl-CoA + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45
-
3-ketohexadecanoyl-CoA
-
Sus scrofa
102
-
3-ketooctanoyl-CoA
-
Sus scrofa
330
-
acetoacetyl-CoA pH 7.0 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sus scrofa