Literature summary for 1.1.1.348 extracted from

Shao, H.; Dixon, R.A.; Wang, X.
Crystal structure of vestitone reductase from alfalfa (Medicago sativa L.) (2007), J. Mol. Biol., 369, 265-276.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Medicago sativa

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.0), 20% (w/v) polyethylene glycol 6000, 0.1 M MgCl2, at 4°C	Medicago sativa

Protein Variants

Protein Variants	Comment	Organism
Y164A	the mutation abolishes enzymatic activity	Medicago sativa
Y164G	the mutation abolishes enzymatic activity	Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3R)-vestitone + NADPH + H+	Medicago sativa	-	(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Medicago sativa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA agarose column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration	Medicago sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-vestitone + NADPH + H+	-	Medicago sativa	(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+	-	ir

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Medicago sativa

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Release 2023.1 (January 2023)