Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
N32D | the mutant exhibits an about 1.3fold increase of catalytic efficiency with NAD+ as cofactor compared to the wild type enzyme | Thermotoga maritima |
N32D/R33I/T34I | the mutant exhibits an about 1.5fold increase of catalytic efficiency with NAD+ as cofactor compared to the wild type enzyme | Thermotoga maritima |
N32D/R33L/T34S | the mutant exhibits an about 0.6fold decrease of catalytic efficiency with NAD+ as cofactor compared to the wild type enzyme | Thermotoga maritima |
N32E/R33I/T34I | the mutant exhibits an about 3.5fold increase of catalytic efficiency with NAD+ as cofactor, with about 64000fold reversal of the coenzyme selectivity from NADP+ to NAD+ compared to the wild type enzyme | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
NAD+ | mutant enzyme N32E/R33I7T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
3.9 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
4 | - |
NAD+ | mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
4.3 | - |
NAD+ | wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
7.5 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | Thermotoga maritima | - |
D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYR9 | cf EC 1.1.1.44 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | - |
Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6PGDH | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.3 | - |
NAD+ | wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
25.4 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
29.3 | - |
NAD+ | mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
32.2 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
47.9 | - |
NAD+ | mutant enzyme N32E/R33I7T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
5.4 | - |
NAD+ | wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
7.3 | - |
NAD+ | mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
8.3 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
19.2 | - |
NAD+ | mutant enzyme N32E/R33I7T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima |