Cloned (Comment) | Organism |
---|---|
gene HMGR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) Rosetta, quantitative realtime-PCR enzyme expression analysis | Alisma plantago-aquatica subsp. orientale |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Alisol B 23-actetate | - |
Alisma plantago-aquatica subsp. orientale |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64600 | - |
- |
Alisma plantago-aquatica subsp. orientale |
66500 | - |
- |
Alisma plantago-aquatica subsp. orientale |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mevalonate + CoA + 2 NADP+ | Alisma plantago-aquatica subsp. orientale | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
r | |
additional information | Alisma plantago-aquatica subsp. orientale | the enzyme is involved in the synthesis of the protostane triterpene Alisol B 23-acetate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alisma plantago-aquatica subsp. orientale | F1DTB9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant solubilized enzyme from Escherichia coli strain BL21(DE3) Rosetta by nickel affinity chromatography and dialysis | Alisma plantago-aquatica subsp. orientale |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies after expression in Escherichia coli strain BL21(DE3) Rosetta | Alisma plantago-aquatica subsp. orientale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mevalonate + CoA + 2 NADP+ | - |
Alisma plantago-aquatica subsp. orientale | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
r | |
additional information | the enzyme is involved in the synthesis of the protostane triterpene Alisol B 23-acetate | Alisma plantago-aquatica subsp. orientale | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64600, about, sequence calculation, x * 66500, recombinant enzyme, SDS-PAGE | Alisma plantago-aquatica subsp. orientale |
More | tertiary structure modelling, overview | Alisma plantago-aquatica subsp. orientale |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl-CoA reductase | - |
Alisma plantago-aquatica subsp. orientale |
HMGR | - |
Alisma plantago-aquatica subsp. orientale |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, reverse reaction | Alisma plantago-aquatica subsp. orientale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at, reverse reaction | Alisma plantago-aquatica subsp. orientale |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Alisma plantago-aquatica subsp. orientale | |
NADPH | - |
Alisma plantago-aquatica subsp. orientale |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alisma plantago-aquatica subsp. orientale | sequence calculation | - |
7.87 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme a key enzyme in the synthetic pathway of protostane triterpenes, including Alisol B 23-acetate and its derivatives | Alisma plantago-aquatica subsp. orientale |