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Literature summary for 1.1.1.34 extracted from

  • Ohto, C.; Muramatsu, M.; Obata, S.; Sakuradani, E.; Shimizu, S.
    Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols (2009), Appl. Microbiol. Biotechnol., 82, 837-845.
    View publication on PubMed

Application

Application Comment Organism
biotechnology overexpression of HMG1 is the most effective among all other genes in both hosts Saccharomyces cerevisiae ATCC 200589 and ATCC 76625 for prenyl alcohol production Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
the HMG1-overexpressing episome (pRS434GAP-HMG1) introduced into 36 type strains purchased from ATCC, and yeast recombinant AURGG101 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P12683
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme in the pathway for production of prenyl alcohols. Almost all Saccharomyces cerevisiae strains tend to produce mainly squalene and low amount of prenyl alcohols. Among these ATCC strains, relatively large amounts of prenyl alcohols in the cultures of two recombinants (ATCC201741 and ATCC 200027). Amounts are quite low compared to the case of ATCC 200589 recombinant. These differences possibly depend on the difference in the squalene synthase activity. If the enzyme activity is weaker in ATCC 200589, the activation of the pathways will result in the accumulation of (E,E)-farnesyl diphosphate, the substrate of the enzyme, and following production of (E,E)-farnesol through hydrolysis of (E,E)-farnesyl diphosphate. Recombinant AURGG101 derived from ATCC 200589 produces large amounts of prenyl alcohols. In particular, (E,E)-farnesol in AURGG101 reaches 35.6 mg/l, which is approximately 4fold higher than that in ATCC 200589. HMG1 expression in strain ATCC 200589 increases the production of squalene, (E)-nerolidol, (E,E)-farnesol, and (E,E,E)-geranylgeraniol, whereas that in ATCC 76625 causes high squalene production, low production of (E,E)-farnesol and (E,E,E)-geranylgeraniol, and no (E)-nerolidol production Saccharomyces cerevisiae ?
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Synonyms

Synonyms Comment Organism
HMG-CoA reductase
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Saccharomyces cerevisiae
HMG1
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Saccharomyces cerevisiae